In-Vivo Mouse Brain Imaging using Three-Photon Fluorescence Adaptive Optics

D. Sinefeld, Fei Xia, Mengran Wang, Tianyu Wang, Chunyan Wu, H. Paudel, Dimitar Ouzounov, T. Bifano, Chris Xu
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Abstract

We demonstrate imaging of deep layers of mouse brain using three-photon fluorescence adaptive-optics based on 1300-nm femtosecond pulses and MEMS spatial-light-modulator. We show significant signal and resolution improvement for neurons imaged both in the cortex and in the hippocampus.
三光子荧光自适应光学在体小鼠脑成像
我们展示了基于1300纳米飞秒脉冲和MEMS空间光调制器的三光子荧光自适应光学对小鼠大脑深层的成像。我们发现在皮层和海马体中成像的神经元信号和分辨率都有显著改善。
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