Zur frage des überlebens von hefestämmen unter flüssigem stickstoff

Abstract

The storage of 140 strains of yeasts in liquid nitrogen at −196°C has been investigated with respect to composition of the freezing medium and survival of cells. The best results were obtained with a freezing mixture containing dimethylsulfoxide, skimmed milk and Ficoll 400, in which 74% of the strains examined showed survival rates of more than 60%. It is not yet possible to give a generally applicable procedure for optimal survival rates of yeasts under liquid nitrogen, since the various strains react very differently to the freezing conditions.