Supplementary Information Text

Abstract

In Vitro Callus Production. Induction of in vitro callus production was performed as previously described (Iwase et al., 2011). Etiolated hypocotyls were excised from mutant and wild type seedlings 4 days after germination. Mutant and wild type explants were cultured for 30 days side-by-side on MS medium supplemented with Gamborg’s vitamins, 2% glucose, 0.05% 2-(N-morpholino)ethanesulfonic acid (MES), pH 5.8 with KOH, 0.8% phytoagar, and the indicated concentrations of NAA and kinetin.