Human melanoma FUHS-1. Description of a new cell line.

Abstract

Presented is a new established cell line, which was cultivated from a melanoma originating from a melanosis praeblastomatosa circumscripta of the leg. Recently subculture No. 105 was reached after a period of 31 month. Medium of primary culture was TC Medium Eagle spinner modified, followed from subculture No. 1 by MEM/Hanks/0.35 g/l NaHCO3. From subculture No. 6 NCTC 135 was applied simultaneously-each respective supplements. The cells grow as monolayer; absolute plating efficiency of the established cell line lies by 800/0; epithelial-like and dendritic melanin-producing cells are prominent. The number of chromosomes is considerably fluctuating; the karyotype of the established cell line lies within the range of subtriploidy. Sizewise a marker in submedian position of centromer is to be compared with one of group.