Uji Aktivitas Ekstrak Daun Mengkudu (Morindra citrifolia Linn) dan Scopoletin secara In-Vitro terhadap Bakteri Tuberkulosis

Indonesian Journal of Pharmacy and Natural Product Pub Date : 2019-04-08 DOI:10.35473/ijpnp.v2i1.192

Estu Mahanani Dhilasari, Idha Kusumawati, Riesta Primaharinastiti

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Abstract

Penyakit tuberkulosis disebabkan oleh bakteri Mycobacterium tuberculosis. Secara tradisional mengkudu digunakan untuk pengobatan tuberkulosis. Scopoletin merupakan komponen utama dalam mengkudu, oleh karena itu scopoletin sering dijadikan marker dalam studi farmakokinetik. Tujuan penelitian ini adalah untuk mengetahui aktivitas anti-Mycobacterium tuberculosis (H37RV) ekstrak daun mengkudu dan scopoletin melalui penentuan Konsentrasi Hambat Minimum (KHM). Uji aktivitas antibakteri dan penentuan KHM dari ekstrak etanol 50% daun mengkudu dilakukan dengan metode dilusi agar dengan konsentrasi 1,0×10-4 µg/ml – 5,1×10-11 µg/ml. Uji aktivitas menunjukan bahwa ekstrak etanol 50% daun mengkudu dapat menghambat pertumbuhan Mycobacterium tuberculosis dengan KHM 4,0×10-6 µg/ml. Sedangkan scopoletin dengan konsentrasi yang setara dengan kandungan pada ekstrak tidak menunjukan aktivitas anti-Mycobacterium tuberculosisnya.Kata kunci: Mengkudu, Scopoletin, tuberkulosisTuberculosis (TB) is a disease caused by a bacterium, Mycobacterium tuberculosis. Morinda citrifolia Linn has been found to kill Mycobacterium tuberculosis. Scopoletin is a major component in Morinda citrifolia Linn, therefore scopoletin often used as markers in studies of pharmacokinetic. This research purpose to determine anti-Mycobacterium tuberculosis (strain H37RV) activity based on the value of Minimum Inhibitory Concentration (MIC) used to ethanolic extracts from Morinda citrifolia Linn leaf and scopoletin. Experiment of anti-Mycobacterium tuberculosis activities tested by well dillution methods with a dose 1,0×10-4 µg/ml – 5,1×10-11 µg/ml . The results showed that ethanolic extract Morinda citrifolia Linn leaf were found to be active to Mycobacterium tuberculosis activity with MIC 4×10-6 µg/ml while scopoletin at the same concentration with extract had no anti- Mycobacterium tuberculosis activity.Keywords: Noni, scopoletin, tuberculosis

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测试活动Mengkudu叶提取物(Morindra citrifolia林)和体外代孕Scopoletin而言对肺结核细菌

结核病是由结核病细菌引起的。传统上用于结核病治疗。Scopoletin mengkudu中的一个主要组成部分,因此Scopoletin药物动力学研究中经常作为标记。anti-Mycobacterium活动研究的目的是了解结核病(H37RV)叶提取物mengkudu和scopoletin通过测定拖住最低浓度(KHM)。抗菌试验活动和决心来KHM银杏叶50%乙醇提取物的mengkudu方法进行稀释,使浓度为1.0×10 - 4µg / ml—5.1×10 - 11µg / ml。测试显示,50%乙醇提取物mengkudu叶活动会阻碍增长和KHM结核病杆菌4.0×10-6µg / ml。而scopoletin浓度为的提取物含量相当于不显示anti-Mycobacterium活动tuberculosisnya。关键词:Mengkudu Scopoletin, tuberkulosisTuberculosis (rs)是a疾病枪舌战bacterium,结核病杆菌。Morinda citrifolia Linn已被发现杀死结核病杆菌。Scopoletin是个少校接口在Morinda citrifolia林,这就是Scopoletin经常在studies of pharmacokinetic过去美国了。这个研究的目的个重大anti-Mycobacterium结核病(H37RV菌株)活动最低价值》改编自Inhibitory双臀(麦克风)习惯ethanolic摘录来自Morinda citrifolia林叶和scopoletin。实验的anti-Mycobacterium结核病活动测试:嗯dillution方法with a剂量1.0×10 - 4µg / ml—5.1×10 - 11µg / ml。The results那里那个ethanolic extract Morinda citrifolia林叶发现to be有源到结核病杆菌和麦克风活动4×10-6µg / ml,当scopoletin at不变双臀反——结核病杆菌与extract已经没有活动。安装:诺尼,scopoletin,结核病

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Indonesian Journal of Pharmacy and Natural Product

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