EFFECT OF AZOTOBACTER SP. AND COW MANURE (CM) ON HYDROCARBON PETROLEUM DEGRADATION RATE BY USING RAMIE PLANT(BOEHMERIA NIVEA)

Abstract

The Petroleum contaminated soil is one of the environment problem which can sharply decrease the soil healthy. A friendly technology that can be used to overcome that problem is Phytoremediation, is a method for removing pollutant by using Plant. In this research was using Ramie (Boehmeria nivea.) as the Phytoremediator. For improving the Rami plant performance to remove hydrocarbon Petroleum was applied Azotobacter sp. and Cow Manure. Azotobacter is the Nitrogen Fixer bacteria that has a role to supply Nitrogen in the rhizophere system. This experiment was focused on to determine the effect of Azotobacter sp. and cow manure (CM) applying on rhizodegradation hydrocarbon petroleum in soil by using Ramie Plants. The experiment was conducted in Green House scale, and the randomized block design was used. The treatments were consist of four treatments (Control; Azotobacter sp; CM dose of 25 g/ 5 kg Soil and CM inoculated by Azotobacter sp.). The results showed that the CM dose of 25 g/ 5 kg soil treatment could significantly increase the population of Azotobacter spp. of 4.8 x 108 CFU/g compared Azotobacter sp. treatment. Azotobacter sp. treatment showed tend to increase the degradation of hydrocarbons rate and dry weight of ramie plants were 0.1047 -day and 40.433 g respectively.