2-Dimensional SPR Detection System Integrated with Molecular Imprinting Polymer Microarrays Using Microfluidic Technology

Abstract

This paper reports a novel microfluidic chip integrated with arrayed molecular imprinting polymer (MIP) films for surface plasmon resonance (SPR) phase imaging of specific bio-samples. The SPR imaging system uses a surface-sensitive optical technique to detect two-dimensional spatial phase variation caused by bio-molecules absorbed on a sensing surface composed of highly-specific MIP films. The developed system has a high resolution and a high-throughput screening capability and has been successfully applied to the analysis of multiple bio-molecules without the need for additional labeling in long-term measuring. The innovative microfluidic chip is capable of transporting a specific amount of multiple bio-samples inside multiple microchannels using micropumps/microvalves to the sensing regions where arrayed MIP films are locally deposited such that highly-sensitive, highly-specific bio-sensing can be achieved.