Analysis and experiment of centrifugal force for microfluidic ELISA CD platform

2010 IEEE EMBS Conference on Biomedical Engineering and Sciences (IECBES) Pub Date : 2010-11-01 DOI:10.1109/IECBES.2010.5742282

F. Ibrahim, P. Jahanshahi, N. Abd Rahman, N. Soin, M. Madou, A. A. Nozari, N. Soin, K. A. Samra, Kameel Abi Samra

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引用次数: 17

Abstract

This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD composed of 2 layers: the substrate layer is made of PMMA and the top layer is laminated with an Adhesive Sealing Films (ASF) or thermal seal. The PMMA substrate was fabricated by CNC micromachining. The ELISA CD consists of 7 reservoirs: waste, detection, serum, conjugate, washing solution, substrate and stopping solution. In the experiment, the reservoirs were filled with colour liquid and the fluid flow behaviour of the liquid in the CD was studied and monitored by a customized CD spin stand system equipped with visualization system. The experimental test results show an average of 9% error when compared with the theoretical calculation of burst frequency for all the reservoirs.

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微流控ELISA CD平台离心力分析与实验

本文介绍了在光盘(CD)平台上对微流控酶联免疫吸附试验(ELISA)进行离心力分析和实验。ELISA CD的设计以离心力为驱动力，毛细力为阻挡屏障。CD由2层组成:基板层由PMMA制成，顶层用胶粘剂密封膜(ASF)或热密封层压。采用数控微加工技术制备PMMA基板。ELISA CD由废液、检测液、血清、偶联物、洗涤液、底物和停液7个贮层组成。在实验中，储层中填充彩色液体，并通过配备可视化系统的定制CD旋转支架系统研究和监测液体在CD中的流动行为。实验测试结果表明，所有储层的冲击频率计算结果与理论计算结果的误差平均为9%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

2010 IEEE EMBS Conference on Biomedical Engineering and Sciences (IECBES)

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