Practical applications of total internal reflection fluorescence microscopy for nanocatalysis

Chengyang Yan, Xuanhao Mei, Xue Gong and Weilin Xu
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Abstract

Fluorescence microscopy has evolved from a purely biological tool to a powerful chemical instrument for imaging and kinetics research into nanocatalysis. And the demand for high signal-to-noise ratio and temporal–spatial resolution detection has encouraged rapid growth in total internal reflection fluorescence microscopy (TIRFM). By producing an evanescent wave on the glass–water interface, excitation can be limited to a thin plane to ensure the measured accuracy of kinetics and image contrast of TIRFM. Thus, this unique physical principle of TIRFM makes it suitable for chemical research. This review outlines applications of TIRFM in the field of chemistry, including imaging and kinetics research. Hence, this review could provide guidance for beginners employing TIRFM to solve current challenges creatively in chemistry.

Keywords: Total internal reflection fluorescence microscopy; Nanocatalysis; Imaging; Kinetics analysis.

Abstract Image

Abstract Image

纳米催化全内反射荧光显微镜的实际应用
荧光显微镜已从纯粹的生物工具发展成为用于纳米催化成像和动力学研究的强大化学仪器。对高信噪比和时空分辨率检测的需求促进了全内反射荧光显微镜(TIRFM)的快速发展。通过在玻璃-水界面上产生蒸发波,可将激发限制在一个薄平面内,从而确保 TIRFM 的动力学测量精度和图像对比度。因此,TIRFM 的这一独特物理原理使其适用于化学研究。本综述概述了 TIRFM 在化学领域的应用,包括成像和动力学研究。因此,本综述可为初学者使用 TIRFM 创造性地解决当前化学领域的难题提供指导:全内反射荧光显微镜;纳米催化;成像;动力学分析。
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来源期刊
Industrial Chemistry & Materials
Industrial Chemistry & Materials chemistry, chemical engineering, functional materials, energy, etc.-
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期刊介绍: Industrial Chemistry & Materials (ICM) publishes significant innovative research and major technological breakthroughs in all aspects of industrial chemistry and materials, with a particular focus on the important innovation of low-carbon chemical industry, energy and functional materials. By bringing researchers, engineers, and policymakers into one place, research is inspired, challenges are solved and the applications of science and technology are accelerated. The global editorial and advisory board members are valued experts in the community. With their support, the rigorous editorial practices and dissemination ensures your research is accessible and discoverable on a global scale. Industrial Chemistry & Materials publishes: ● Communications ● Full papers ● Minireviews ● Reviews ● Perspectives ● Comments
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