Keberkesanan Antibiotik Titisan Mata Gentamicin Terhadap Sista Acanthamoeba spp. Isolat Klinikal

Abstract

Acanthamoeba spp. boleh menyebabkan keratitis kepada manusia, khasnya kepada pemakai kanta sentuh yang tidak menitikberatkan aspek higen kanta sentuh. Kajian ini bertujuan untuk mengkaji keberkesanan antibiotik titisan mata Gentamicin terhadap sista Acanthamoeba isolat klinikal iaitu HKL 5, HKL 11, HKL 32, dan HKL 51. Kajian dimulakan dengan melakukan suspensi sista terhadap keempat-empat isolat klinikal tersebut. Mikropencairan terhadap antimikrob dilakukan dan kemudiannya suspensi sista ditambah, seterusnya dieramkan selama 24 jam pada 30oC sebelum dipindahkan ke atas plat agar bukan nutrien (NNA) yang dilapisi E. coli matian haba. Plat agar diperhatikan setiap hari sehingga hari yang ke-14 untuk mengesan sebarang kehadiran trofozoit di bawah mikroskop inverted. Kehadiran trofozoit Acanthamoeba menunjukkan bahawa antibiotik ujian tidak berkesan terhadap sista Acanthamoeba. Gentamicin berkesan terhadap sista Acanthamoeba, dengan nilai purata sistisidal minimum (MCC) 1453.1 μg/ml. Gentamicin merupakan antibiotik yang boleh digunakan dalam rawatan keratitis Acanthamoeba memandangkan kepada keberkesanannya terhadap sista Acanthamoeba isolat klinikal dalam kajian ini. __________________________________________________________________________ Acanthamoeba spp. may cause keratitis in humans especially amongst the contact lens wearers who do not practice good hygienic care of contact lenses. This study aims to evaluate the effectiveness of Gentamicin on four clinical isolates of Acanthamoeba cysts from Kuala Lumpur Hospital consisting HKL 5, HKL 11, HKL 32, and HKL 51. Cysts suspensions of all 4 clinical isolates of Acanthamoeba were prepared. Microdilution of antimicrobial was prepared and cyst suspension was added and later incubated at 30oC for 24 hours before being transferred onto non-nutrient agar (NNA) plates seeded with heat-killed E.coli. The plates were observed daily until day 14 to detect the presence of trophozoites under inverted microscope. The presence of trophozoite stage indicates ineffectiveness of the tested antibiotic towards Acanthamoeba cysts. Gentamicin was found to be effective against Acanthamoeba cysts with MCC mean value of 1453.1 μg/ml. Gentamicin could be used for the treatment of Acanthamoeba keratitis as it exhibited cysticidal effect on all isolates of Acanthamoeba cysts in this study. Keywords: Topical antibiotic eyedrop, Gentamicin, Acanthamoeba, Malaysia