Methylene blue as a photosensitizer in the photodynamic therapy of breast cancer cell lines

Abstract

We compared MB-PDT effect on two breast cancer cell lines: MDA-MB-231 from a triple negative adenocarcinoma, and T47D from a ductal cell tumor of woman. Methylene blue concentrations at 5, 10 and 20 μM and red light doses of 20, 40 and 60 J/cm2 were employed. Cell viability was evaluated with the MTT test, obtaining around 80% of inhibition with 20 μM and 60 J/cm2 of light dose.