Lysosomal or brush border enzymuria in kidney enzyme activities in seronegative rheumatoid arthritis

D. Spasovski
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Abstract

Introduction: To determine the effects of non-treated seronegative rheumatoid arthritis (RA) on proximal renal tubule, sensitivity of Alanine aminopeptidase (AAP), g-glutamyltransferase (g--GT), β2 microglobulin in urine β2M), as well as relation with rheumatoid factor (RF) and C-Reactive protein (CRP), DAS 28 disease activity index. Methods: RF was determined by agglutination test (Latex RF test, while kinetic methods were used for determination of Alanine aminopeptidase (AAP) and g-glutamyltransferase (g-GT), as well as MEIA (Micro particle Enzyme Immunoassay) to determine β2 microglobulin in urine. Samples (serum and urine) of 70 participants were examined (35 RA not treated, 35 health control group). Results: In 35 RF negative RA, AAP enzymuria was present in 12 (34.28%) patients, g-GT was present in 7 patients (20%), while β2 microglobulin was present in 3 patients (8.57%). In the healthy control group, 4 patients showed AAP positivity (11.42%), 2 patients g-GT positivity (5.71%) and 1 patient showed presence of β2 microglobulin in urine (2.85). RF was not present in any patient (0%). Conclusion: AAP has a higher sensitivity of g-GT and b2 microglobulin in the detection of asymptomatic renal lesions in non treated seronegative RA.
血清阴性类风湿关节炎肾酶活性中的溶酶体或刷边酶血症
前言:探讨未治疗的血清阴性类风湿性关节炎(RA)对近端肾小管的影响、丙氨酸氨基肽酶(AAP)、g-谷氨酰转移酶(g- GT)、尿中β2微球蛋白(β2M)的敏感性,以及与类风湿因子(RF)、c反应蛋白(CRP)、DAS 28疾病活动性指数的关系。方法:采用凝集试验(Latex RF试验)测定血清中β2微球蛋白含量,采用动力学法测定丙氨酸氨基肽酶(AAP)和g-谷氨酰转移酶(g-GT)含量,采用微粒子酶免疫分析法(MEIA)测定尿液中β2微球蛋白含量。70名参与者的血清和尿液样本(35名RA未治疗,35名健康对照组)被检查。结果:35例RF阴性RA中,AAP酶血症12例(34.28%),g-GT 7例(20%),β2微球蛋白3例(8.57%)。健康对照组AAP阳性4例(11.42%),g-GT阳性2例(5.71%),尿中β2微球蛋白1例(2.85)。所有患者均未出现RF(0%)。结论:AAP检测g-GT和b2微球蛋白对未治疗的血清阴性RA无症状肾损害有较高的敏感性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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