The comparison of viral antibody titers of acid-precipitated and non-precipitated mouse ascitic fluids.

Abstract

Mouse immune ascitic fluid has become a primary source of antibody for diagnostic, reference, and research work in many virus laboratories. The inherent disadvantage of ascitic fluid is that it repeatedly forms clots and subsequently loses volume. The acid-precipitation method of Chiewsilp and McCown eliminates the clot formation and does not appreciably alter the antibody titers for several arboviruses, varicella, rabies and influenza viruses.