Simultaneous biophoton measurement of control and fluoride stressed seedlings samples

E. Bertogna, E. Conforti, C. Gallep
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引用次数: 2

Abstract

A series of 10 germinations experiments with duplicated wheat seedlings samples - one imbibed in sodium fluoride (NaF, EC50) and another in water - have their spontaneous, ultra-weak photon emission measured simultaneously for 24-h, at the 4th day of germination, in two identical measurement systems. The UPE profiles of acute stressed sprouts present initially higher increase but after 6 hours a fast decrease. These variations for the profiles local angular coefficient are so correlated to germination parameters - roots elongation and biomass gain - with clear difference between the stressed and control samples. Variations of such methodology can be developed for quick and reliable analysis of toxicity in germination tests.
对照和氟胁迫幼苗样品的同步光子测量
在两个相同的测量系统中,在萌发的第4天,同时测量了10个重复的小麦幼苗样品的自发的、超弱的光子发射,其中一个样品在氟化钠(NaF, EC50)中吸收,另一个在水中吸收。急性应激芽苗菜的UPE曲线呈现出初期较高的上升趋势,但6 h后迅速下降。这些剖面局部角系数的变化与发芽参数(根系伸长和生物量增加)密切相关,在胁迫和对照样品之间存在明显差异。这种方法的变化可以开发快速和可靠的分析发芽试验中的毒性。
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