Ethyl acetate extract of Terminalia arjuna bark shows antioxidant activity and protects against oxidative stress in rat liver mitochondria

Abstract

The antioxidant properties of Terminalia arjuna (TA) bark have been identified in this present study. The antioxidant properties of ethyl acetate extract of TA bark were established by DPPH radical scavenging activity, reducing power, H2O2 scavenging activity, scavenging activity of hydroxyl radical, superoxide radical, and nitric oxide radical. This present study also showed the protective effects of ethyl acetate extract against copper-ascorbate-induced oxidative stress in rat liver mitochondria in vitro. The ethyl acetate extract of TA barkaltered the content of reduced glutathione (GSH), levels of lipid-peroxidation (LPO), and protein carbonylation (PCO) (P<0.001) against copper-ascorbate treated rat liver mitochondria, in vitro. Thus, the results indicate that the ethyl acetate extract of TA bark can be considered as a therapeutic agent in the future.