PENGARUH PENAMBAHAN SARI WORTEL DALAM PENGENCER SITRAT KUNING TELUR TERHADAP KUALITAS SPERMATOZOA KAMBING BLIGON (The effect of carrot juice supplementation in citrate - egg yolk extender on spermatozoa quality of bligon goat)

JURNAL NUKLEUS PETERNAKAN Pub Date : 2020-12-17 DOI:10.35508/NUKLEUS.V7I2.3152

Maria Epifania Barek, Kirenius Uly, T. M. Hine, W. Nalley, H.L.L. Belli

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引用次数: 2

Abstract

This study aims to determine the effect of adding various doses of carrot juice into citrate-egg yolk diluents (C-EY) on bligon buck semen quality. Semen was collected twice a week using an artificial vaginal method of three buck with normal body condition and reproductive organs. Semen was 70% of motility, konsentration ≥1000x106mL and abnormality ≤ 15 diluted with C-EY added with carrot juice at a concentrations: 0% (CJ-0), 10% (CJ-10), 12.5% (CJ-12,5), 15% (CJ-15), 17.5% (CJ-17,5), and 20% (CJ-20), and stored in a refrigerator at 3-5o C. The quality of spermatozoa was evaluated every 24 hours. The Variables measured were spermatozoa motility, viability, and abnormalities. The results showed that spermatozoa preserved by C-EY supplemented by 17.5% carrot juice (CJ-17.5) had a higher quality (P<0.05) compared to the other four treatments, with motility (49.04 ± 1.0%), viability (55.70 ± 1.22%), dan abnormalities (4.57 ± 0.88 %) on the 4th day of storage. This study concludes that 17.5% carrot juice into citrate eggyolk extender improves the bligon buck semen

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本研究旨在研究在柠檬酸蛋黄稀释液中加入不同剂量的胡萝卜汁对雄鹿精液质量的影响。每周采集两次精液，采用三雄人工阴道法，身体状况和生殖器官正常。精液活动力70%，浓度≥1000x106mL，异常≤15，用C-EY加入胡萝卜汁稀释，浓度分别为0% (CJ-0)、10% (CJ-10)、12.5% (cj -12,5)、15% (CJ-15)、17.5% (cj -17,5)、20% (CJ-20)，保存于3- 50℃的冰箱中，每24小时评价一次精子质量。测量的变量是精子活力、活力和异常。结果表明，添加17.5%胡萝卜汁(jc -17.5)的C-EY保存的精子在保存第4天的活力(49.04±1.0%)、活力(55.70±1.22%)、异常率(4.57±0.88%)高于其他4种处理(P<0.05)。本研究表明，在柠檬酸蛋液中添加17.5%的胡萝卜汁对雄鹿精液有改善作用

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JURNAL NUKLEUS PETERNAKAN

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