Effect of pH along with other variables on physical properties of collagen nanofibers

Abstract

In this study the effects of temperature and polymer concentration were examined on the formation of neutral and acidic collagen nanofibers using benign solution. Neutral and acidic electrospun collagen nanofibers diameter were in the range of 90 to 220 nm and 160 to 310 nm respectively. Moreover, the acidic collagen solution had more stable jets and less bead formation was observed.` Fourier transform infrared spectroscopy showed the maintenance of the collagen triple helix at the temperature lower than 25°C for only neutral samples and the transition to the random coil in acidic collagen. In consequence, regarding the application of the scaffold factors such as pH, concentration of the polymer and temperature can be altered.