Holographic Red Blood Cell Image Analysis

Abstract

Digital holographic microscopy (DHM) is a well-suited technique for studying red blood cell (RBC) since it is a perfect phase object. Also, DHM is a contactless and non-invasive method thus the sample can be studied without damaging the sample. In this paper, we show different analysis about RBC's quantitative phase images (QPI) provided by DHM. The parameters are related to the shape of RBC such as volume, membrane surface area, projected surface area, sphericity index, RBC's radius and sphericity coefficient. The application of studying RBC by DHM is not limited to the shape of RBC and biochemical property such as hemoglobin content can also be analyzed. The images are related to the healthy RBC sample, and after imaging, segmentation can provide us several single cells. These parameters can be helpful for red blood cell experts and hematologist.