A microcapsule array for quantitative fluorescence detection of copper ions based on functional nucleic acid

Enqi He, Liangyuan Cai, D. Guo, Yinglin Zhou, Xinxiang Zhang, Zhihong Li
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Abstract

This paper reports a portable, low-cost, user-friendly and ultrasensitive microcapsule array for quantitative fluorescence detection of copper ions based on functional nucleic acid strategy. Since the reagents are pre-sealed through ice printing technique and stored in frozen microcapsules before application, long-term stability of this device is guaranteed. Trace Cu2+ sample is simply injected into the thawy microcapsule by microsyringe, and after 15 minutes the fluorescence results are shown under an LED transilluminator. This assay shows great sensitivity with the minimum detection limit of 100 nM (100 fmol/μL) and specificity. Furthermore, it can be applied into other assays based on DNA fluorescence detection.
一种基于功能核酸的铜离子定量荧光检测微胶囊阵列
本文报道了一种基于功能核酸策略的便携式、低成本、用户友好、超灵敏的铜离子荧光定量检测微胶囊阵列。由于试剂在使用前通过冰印技术进行预密封,并储存在冷冻微胶囊中,因此保证了该设备的长期稳定性。微量Cu2+样品通过微注射器注入融化的微胶囊中,15分钟后在LED透光灯下显示荧光结果。该方法灵敏度高,最低检出限为100 nM (100 fmol/μL),特异性强。此外,它可以应用于其他基于DNA荧光检测的分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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