Measurements of ligament tissue cross-sectional area

D.J. Lanzendorf, M. Murphy, R. Mann
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引用次数: 2

Abstract

An experimental method utilizing a high-speed computerized image analysis system to measure the cross-sectional area of ligament tissue was developed. The method consists of flash-freezing ligament tissue in liquid nitrogen and then sectioning the tissue at discrete locations along its length. A nondestructive casting technique was also developed to determine the cross-sectional area of ligament specimens prior to sectioning. Images of individual cross-sections for both ligament and cast sections were acquired using a high-resolution video-camera system. A comparison study was performed for an average area value of ligament cross sections and an average area value for cast cross sections. The variations between these are within 8.3%. A section-by-section comparison of ligament versus casting was also performed. In most cases, the variations between ligament section and cast section was less than 10%. The casting technique utilizing the image analysis system in an accurate, nondestructive way to determine the cross-sectional area of ligament tissue. It does not assume a shape for the area and can detect concavities on the surface of the tissue.<>
测量韧带组织横截面积
提出了一种利用高速计算机图像分析系统测量韧带组织横截面积的实验方法。该方法包括在液氮中快速冷冻韧带组织,然后沿其长度在离散位置切片。一种无损铸造技术也被开发,以确定韧带标本的横截面积之前的切片。使用高分辨率摄像机系统获得韧带和铸型切片的单个横截面图像。对韧带横截面的平均面积值与铸型横截面的平均面积值进行了比较研究。两者之间的差异在8.3%以内。还进行了韧带与铸造的逐段比较。在大多数情况下,韧带切片与铸型切片之间的差异小于10%。该铸造技术利用图像分析系统以一种准确、无损的方式来确定韧带组织的横截面积。它不假设该区域的形状,并且可以检测组织表面的凹陷。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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