{"title":"Measurements of ligament tissue cross-sectional area","authors":"D.J. Lanzendorf, M. Murphy, R. Mann","doi":"10.1109/NEBC.1988.19333","DOIUrl":null,"url":null,"abstract":"An experimental method utilizing a high-speed computerized image analysis system to measure the cross-sectional area of ligament tissue was developed. The method consists of flash-freezing ligament tissue in liquid nitrogen and then sectioning the tissue at discrete locations along its length. A nondestructive casting technique was also developed to determine the cross-sectional area of ligament specimens prior to sectioning. Images of individual cross-sections for both ligament and cast sections were acquired using a high-resolution video-camera system. A comparison study was performed for an average area value of ligament cross sections and an average area value for cast cross sections. The variations between these are within 8.3%. A section-by-section comparison of ligament versus casting was also performed. In most cases, the variations between ligament section and cast section was less than 10%. The casting technique utilizing the image analysis system in an accurate, nondestructive way to determine the cross-sectional area of ligament tissue. It does not assume a shape for the area and can detect concavities on the surface of the tissue.<<ETX>>","PeriodicalId":165980,"journal":{"name":"Proceedings of the 1988 Fourteenth Annual Northeast Bioengineering Conference","volume":"38 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1988-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the 1988 Fourteenth Annual Northeast Bioengineering Conference","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NEBC.1988.19333","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
An experimental method utilizing a high-speed computerized image analysis system to measure the cross-sectional area of ligament tissue was developed. The method consists of flash-freezing ligament tissue in liquid nitrogen and then sectioning the tissue at discrete locations along its length. A nondestructive casting technique was also developed to determine the cross-sectional area of ligament specimens prior to sectioning. Images of individual cross-sections for both ligament and cast sections were acquired using a high-resolution video-camera system. A comparison study was performed for an average area value of ligament cross sections and an average area value for cast cross sections. The variations between these are within 8.3%. A section-by-section comparison of ligament versus casting was also performed. In most cases, the variations between ligament section and cast section was less than 10%. The casting technique utilizing the image analysis system in an accurate, nondestructive way to determine the cross-sectional area of ligament tissue. It does not assume a shape for the area and can detect concavities on the surface of the tissue.<>