Expression of modified VHb gene and fused insectidal gene in tobacco

Abstract

The highly-efficient plant binary expression vector pGBIF4ABCVHB was constructed by applying the artificially-modified-synthesized vgbM gene and fusedly-insectidal genes(GFMcryIA and CPTI ). Transgenic tobacco(Nicotiana tabacum) plants were obtained by Agrobacterium mediated transformation, 32 of them were identified that the bivalent genes were inserted into the tobacco genome by PCR amplification detection and Southern blotting analysis. Western blotting analysis proved that vgbM gene was expressed in the transgenic plants. Toxicity assay indicated that fused insectidal genes were expressed activity of pesticadal toxin protein.The net weight of transgenic tobacco plants exceeded 8% of that of non- transgenic ones. Compared to non-transgenic tobacco plants, the transgenic ones were high-yield varieties of insect-resistance.