Determination of ABO Blood Groups from Human Salivary Stain by the Mixed Agglutination Reaction Method Using an OHP Film

Abstract

For ABO blood typing of human salivary stains, a new mixed agglutination reaction method was devised, in which, OHP film was used as antigen-antibody reaction plate. By this method a series of examination procedures such as extraction of blood group substances, heating-fixation of the substance and incubation with monoclonal antibodies could be performed successively on the OHP film. For the secretor's salivary stain samples, specific agglutination reaction which presents an agglutination circle was observed. ABO blood typing from secretor's salivary stains was correctly performed by this method.