Production of biological hydrogen and bacterial carotenoids with Rhodobacter sphaeroides O.U.001 in a biorefinery concept

Kübra Daniş, Buse Nur Bi̇ngöl, Gökhan Kars
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Abstract

In this study, the goal was to produce biohydrogen and bacterial carotenoids with Rhodobacter sphaeroides O.U.001, a purple non-sulfur photosynthetic bacterium, utilizing sugar beet molasses in the context of biorefinery. First, media with different sugar concentrations (10 g/L, 20 g/L, 30 g/L, 40 g/L, 50 g/L) were prepared for bacterial growth. Then, hydrogen production was carried out using these media in anaerobic conditions in 100 ml bioreactors. After hydrogen gas was collected from the bioreactors, carotenoid extraction was performed from the remaining bacteria. As a result of the analyzes, it was found that the amount of biohydrogen and the amount of bacterial carotenoids obtained were inversely proportional to the increased sugar concentrations. The maximum hydrogen formation was detected in the medium containing 10 g/L of sugar (19.18 mL). According to the results of gas chromatography analysis, the quantity of hydrogen in the total gas was found to be around 23.6%. The highest yield of carotenoids was again obtained from bacteria reproduced in a medium containing 10 g/L of sugar (3.12 mg/g, carotenoid/dry biomass). As a conclusion, this study provides an example for the successful realization of two high value-added products within a biorefinery approach by using molasses obtained at an affordable cost.
在生物炼制概念中利用球形红杆菌O.U.001生产生物氢和细菌类胡萝卜素
在这项研究中,目标是利用生物炼制背景下的甜菜糖蜜,利用紫色无硫光合细菌球形红杆菌O.U.001生产生物氢和细菌类胡萝卜素。首先,制备不同糖浓度的培养基(10 g/L、20 g/L、30 g/L、40 g/L、50 g/L)供细菌生长。然后,利用这些培养基在厌氧条件下在100 ml生物反应器中进行制氢。从生物反应器中收集氢气后,从剩余细菌中提取类胡萝卜素。分析结果发现,生物氢的含量和细菌类胡萝卜素的含量与糖浓度的增加成反比。在含糖量为10 g/L (19.18 mL)的培养基中,产氢量最大。根据气相色谱分析结果,发现总气体中氢气的含量约为23.6%。在含有10 g/L糖(3.12 mg/g,类胡萝卜素/干生物量)的培养基中繁殖的细菌再次获得了最高的类胡萝卜素产量。作为结论,本研究为生物炼制方法中成功实现两种高附加值产品提供了一个例子,该方法使用以可承受的成本获得的糖蜜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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