Cytochrome c release caused by Pulsed Electric Fields (PEFs) with different time durations

Abstract

Multilayer Dielectric Sphere Model (MDSM) is used for analyzing responses of cells to Pulsed Electric Fields (PEFs), and cytochrome c release is significant effect of PEFs when mitochondrial substructures are taken into account. Numerical results of MDSM model explain and verify the reported experimental results on Intracellular Electro-manipulation (IEM). It is proposed that two-step process of cytochrome c release can be implemented by bioelectric impulse effect on mitochondria.