Development of MNPs Based Enzyme Immuno-Sorbent Analysis for the Determination of Organophosphorus Pesticides in Milk

Abstract

Magnetic nanoparticles were synthesized and functionalized with (3-aminopropyl)triethoxysilane. Sheep polyclonal-anti-paraoxon and antidichlorvos antibodies were produced. Dichlorvos-cationized ovalbumin-HPR and paraoxon-ovalbumin-HPR conjugates were prepared and characterized. The optimal conditions for antibody immobilization were determined. Enzyme immunosorbent assay based on Magnetic Nanoparticles (MNPs) for the determination of pesticides was developed. A comparison of the developed modified MNPs-based ELISA with the conventional ELISA was performed. The effect of protein, pH, and fats in milk samples on the analytical characteristics of the MNPs-based ELISA was investigated. In order to validate the results obtained from the MNPs-based ELISA method, experiments with HPLC were performed.