Exploration of dysregulated lncRNA-mRNA network from the RNA-seq data of rats induced by three different synthetic cytotoxic compounds

Abstract

Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play important roles in initiation and development of human diseases. However, the mechanism of the targets regulated by lncRNA remains unclear. In this study, we performed a multi-step computational analysis to construct dysregulated lncRNA-mRNA networks for the rats’ RNAseq data induced by three different synthetic cytotoxic compounds (CARBON TETRACHLORIDE, CHLOROFORM, THIOACETAMIDE). We systematically integrated lncRNA and mRNA expression profiles and lncRNA-mRNA regulatory interactions. The constructed interaction network exhibited biological network characteristics, and functional analysis demonstrated that the networks were specific for inducing synthetic compounds. Additionally, we identified some lncRNA-mRNA modules. This study will provide us new insight into lncRNA-mRNA regulatory mechanisms involved in rats induced by three different synthetic cytotoxic compounds and will facilitate the discovery of candidate diagnostic and prognosis biomarkers for related diseases.