DYNAMICS OF THE CONCENTRATION OF CHLOROPHYLL A AND B-PHYCOERYTHRIN IN CULTURE PORPHYRIDIUM PURPUREUM IN CONDITIONS OF LIGHT AND CARBON LIMITATION

V. Klochkova, A. Lelekov, I. Gudvilovich
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Abstract

The effect of light intensity and carbon flux on the production of chlorophyll a and B-phycoerythrin, as well as their ratios in the batch culture of Porphyridium purpureum, has been studied. It is shown that with an increase in light intensity (by 2 times) and air supply speed (by 2 times), the value of maximum productivity increases by almost 2 times, the concentration of chl a – by 1.8 times, and B-PE – by 1.6 times. The content of chlorophyll a and B-phycoerythrin in all experimental variants on the 3rd – 4th day of the experiment (the beginning of the linear growth phase) reached the maximum value. With light limiting, the content of chl a and B-PE in the biomass does not change, however, with high light intensity, a decrease is observed in the linear growth phase. The ratio of B-PE/chl a with different light and carbon support in the experiment practically does not change and averages 12.8.
光限碳条件下紫斑卟啉叶绿素a和b -藻红蛋白浓度的动态变化
研究了不同光照强度和碳通量对紫斑卟啉(Porphyridium purpureum)分批培养中叶绿素a和b -藻红蛋白产量及其比例的影响。结果表明,光照强度增加2倍,送风速度增加2倍,最大生产率增加近2倍,chl -浓度增加1.8倍,B-PE -浓度增加1.6倍。叶绿素a和b -藻红蛋白含量在试验第3 ~ 4天(线性生长期开始)达到最大值。在限光条件下,生物量中chl a和B-PE含量没有变化,但在高光照条件下,线性生长期chl a和B-PE含量有所下降。实验中不同光碳载体下B-PE/chl a的比值基本不变,平均为12.8。
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