Evaluation of soma-clonal variation in CP-671026 mutant of sugarcane (Saccharum officinrum L) through morphological techniques

Zohra Fatima Memon, Shala Baloch, Muharam Ali, I. A. Khan, A. Khaskheli, S. Yasmine, S. Chachar, Zareen Qambrani, Bilqees Magsi, Sayyed Zakir Hussain Shah
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Abstract

Present studies were conducted on evaluation of soma-clonal variation of CP67-1026 mutant through morphological procedures with the aims at discussing how soma-clonal variations can be analyzed through morphological approaches in gaining knowledge about the underlined regulatory controlled mechanisms in plants. In vitro experiments were conducted in the Laboratory of Nuclear Institute Atomic Energy (NIA), Tandojam during the year of 2021. The results so far achieved opened further window for deep analysis with reference to functional analysis of soma-clonal variated plants with different approaches. Results on the performance for soma-clonal variation in sugarcane variety NIA-2010 and their soma-clones revealed the best performance of varieties, which showed that the plant height (302.33cm) was observed in SC-3 and the lowest performance was examined in SC-2 that was approximately (154.33cm). The consequences of varieties and diverse parameters indicated that the highest number of internodes plant-1 were recorded (38) in SC-3 and lowest number of internodes plant-1 were accomplished (23) in SC-1. The output of different features indicated that highest number of tillers plant-1 was observed (8.02) in SC-3, the lowest number of tillers plant-1 (4.33) were noted in SC-4 and 8 as well. The consequences of soma-clones indicated that maximum weight stool-1 (kg) was observed (9.67kg) in SC-3 and minimum weight stool-1 (kg) was showed (5.83) in SC-1. The output of different physiological features indicated that highest values were remarkably noticed of brix (%) (19.83%) in SC-13, sucrose (%) (13.4%) in SC-3, fiber (%) (18.92g) in SC-3 whereas the lowest fiber (%) (16.58g) were seen in SC-11. The results of various agronomic parameters such as germination % observed (82.01%) in SC-6, commercial cane sugar percentage (CCS %) showed (9.1%) in SC-9, sugar recovery (%) (8.56%) in SC-8, purity (%) (85.27%) in SC-8, cane yield (tonnes per hectare) (96.67 tonnes per hectare) in SC-3, sugar yield (tonnes per hectare) (7.95 tonnes per hectare) in SC-3 for the assessment of morphological constitutions of NIA-2010 genotype and their soma-clone
甘蔗(Saccharum officinrum L) CP-671026突变体体细胞克隆变异的形态学评价
本研究通过形态学方法对CP67-1026突变体的体细胞克隆变异进行了评价,旨在探讨如何通过形态学方法分析体细胞克隆变异,从而了解植物中强调的调控机制。体外实验于2021年在坦多贾姆原子能研究所(NIA)实验室进行。这些结果为借鉴不同方法对体细胞克隆变异植物的功能分析打开了进一步深入分析的窗口。结果表明,甘蔗品种NIA-2010及其体细胞无性系的变异表现最好,其中SC-3的株高为302.33cm, SC-2的株高最低,约为154.33cm。品种和参数的结果表明,SC-3的节间株数最多(38),SC-1的节间株数最少(23)。各性状的输出结果表明,SC-3分蘖数最多(8.02),SC-4和sc - 8分蘖数最少(4.33)。体细胞克隆结果表明,SC-3的最大重量(kg)为9.67kg, SC-1的最小重量(kg)为5.83 kg。不同生理特征的输出结果表明,SC-13的糖度(%)(19.83%)最高,SC-3的蔗糖(%)(13.4%)最高,SC-3的纤维(%)(18.92g)最高,SC-11的纤维(%)最低(16.58g)。利用各种农艺参数,如SC-6的发芽率(82.01%)、SC-9的商品蔗糖率(CCS %)(9.1%)、SC-8的糖回收率(%)(8.56%)、SC-8的纯度(%)(85.27%)、SC-3的甘蔗产量(每公顷96.67吨)、SC-3的糖产量(每公顷7.95吨)等,对NIA-2010基因型及其体细胞克隆的形态构成进行了评价
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