Serotonin enrichment of rice endosperm by metabolic engineering

IF 6 1区农林科学 Q1 AGRONOMY

Crop Journal Pub Date : 2023-12-01 DOI:10.1016/j.cj.2023.07.003

Qingqing Yang , Yan Tan , Ying Ye , Dongsheng Zhao , Qiaoquan Liu

{"title":"Serotonin enrichment of rice endosperm by metabolic engineering","authors":"Qingqing Yang , Yan Tan , Ying Ye , Dongsheng Zhao , Qiaoquan Liu","doi":"10.1016/j.cj.2023.07.003","DOIUrl":null,"url":null,"abstract":"<div>In animals, serotonin is a neurotransmitter and mood regulator. In plants, serotonin functions in energy acquisition, tissue maintenance, delay of senescence, and response to biotic and abiotic stresses. In this study, we examined the effect of serotonin enrichment of rice endosperm on plant growth, endosperm development, and grain quality. To do so, TDCs and T5H were selected as targets for serotonin fortification. Overexpression of TDC1 or TDC3 increased serotonin accumulation relative to overexpression of T5H in rice grain. Transgenic lines of target genes driven by the Gt1 promoter showed better field performance than those driven by the Ubi promoter. Overexpression of T5H showed little effect on plant growth or grain physicochemical quality. In neuronal cell culture assays, serotonin induced neuroprotective action against apoptosis. Breeding of rice cultivars with high serotonin content may be beneficial for health and nutrition.</div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123000995/pdfft?md5=dea353c70e8e6d9e927575ac1fbd4cc6&pid=1-s2.0-S2214514123000995-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123000995","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

In animals, serotonin is a neurotransmitter and mood regulator. In plants, serotonin functions in energy acquisition, tissue maintenance, delay of senescence, and response to biotic and abiotic stresses. In this study, we examined the effect of serotonin enrichment of rice endosperm on plant growth, endosperm development, and grain quality. To do so, TDCs and T5H were selected as targets for serotonin fortification. Overexpression of TDC1 or TDC3 increased serotonin accumulation relative to overexpression of T5H in rice grain. Transgenic lines of target genes driven by the Gt1 promoter showed better field performance than those driven by the Ubi promoter. Overexpression of T5H showed little effect on plant growth or grain physicochemical quality. In neuronal cell culture assays, serotonin induced neuroprotective action against apoptosis. Breeding of rice cultivars with high serotonin content may be beneficial for health and nutrition.

查看原文本刊更多论文

通过代谢工程富集水稻胚乳中的羟色胺

在动物体内，血清素是一种神经递质和情绪调节剂。在植物中，血清素具有获取能量、维持组织、延缓衰老以及应对生物和非生物胁迫的功能。在这项研究中，我们考察了水稻胚乳中富含血清素对植物生长、胚乳发育和谷物品质的影响。为此，我们选择了 TDCs 和 T5H 作为血清素强化的靶标。相对于过表达 T5H，过表达 TDC1 或 TDC3 会增加血清素在水稻谷粒中的积累。由 Gt1 启动子驱动的目标基因转基因品系的田间表现优于由 Ubi 启动子驱动的品系。过量表达 T5H 对植物生长和谷物理化品质的影响很小。在神经元细胞培养试验中，5-羟色胺可诱导神经保护作用，防止细胞凋亡。培育血清素含量高的水稻品种可能对健康和营养有益。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

9.90

自引率

3.00%

发文量

638

审稿时长

41 days

期刊介绍： The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.