Self-assembled mini-SPR for detecting the stability of the bio-membrane

Abstract

Supported Bilayer Lipid Membrane (s-BLM) affords a great direction to study the lipid membrane. It becomes a key to study the stability of s-BLM. The study is adopting a method with optics to detect data not electronics, because it can avoid the disturbance which is brought by the electric charge. Based on the basic principles of SPR, we assembled a mini-SPR equipment, and it has the accuracy fitting for our works. To adjust the angle of the polarizer and move the fiber, we can make the spectrogram that membrane is coupled on the surface of the gold slice. Comparing to the reference spectrogram, we can make a certain relationship between the light absorbed maximum and the quality of the phospholipid molecules coupling on the surface. It can token the resident time with the time which the detected spectrogram resumes to the reference one. And the resonance angle has a definite shift. We can assess the stability of phospholipid BLM with the three references. The mini-SPR equipment can easily change the parts and the parameter with the up-and-down equipment, the movement of the fiber and replacing the triangular prism plated the thin metal. So we can explore and find the conditions of the stability of BLM effectively with it[1].