In vitro evaluation of ionizing radiation effects in bone tissue by FTIR spectroscopy

Abstract

We verified the changes promoted by ionizing radiation in bone tissue using FTIR. Samples of bovine bone were irradiated using Cobalt-60 with 0.01kGy, 0.1kGy, 1kGy, 15kGy and 75kGy. The effects of ionizing irradiation on chemical structure of bone, were studied considering the sub-bands of amide I, the crystallinity index and relation of organic and inorganic materials. ATR-FTIR spectroscopy showed changes in organic components and in hydroxyapatite crystals organization. High correlation with statistical significance was observed between (amideIII+collagen)/ ν1,ν3PO4, crystallinity and mechanical properties of the samples.