[A new insulin radioimmunotest and its use in the determination of anti-insulin antibodies].

Abstract

Acetylation of insulin with 3H-acetic anhydride resulted in the predominant 3H-labeling at the aminoterminal position of the B-chain (B1). The labeled insulin was unchanged in its immunologic reactivity as compared to native insulin. It was used in a second antibody radioimmunoassay for the detection of antiinsulin-antibodies in insulin-dependent patients with Diabetes mellitus.