Effect of dichloromethylene diphosphonate on morphology, enzyme activity, and ash content of bones of thyroparathyroidectomized rats.

Calcified Tissue Research Pub Date : 1978-05-26 DOI:10.1007/BF02010760

S E Weisbrode, C C Capen, C B Pendley

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引用次数: 1

Abstract

Dichloromethylene diphosphonate (Cl2MDP) was given at doses of 4 mg/kg and 10 mg/kg daily for 7 days to adult thyroparathyroidectomized rats fed a low calcium diet. Primary metaphyseal trabeculae in Cl2MDP-treated rats were more numerous and longer than in controls. The light and electron microscopic appearance of osteoblasts, osteocytes and osteoclasts were unaltered by Cl2MDP. Bone alkaline phosphatase was significantly elevated in rats given Cl2MDP but adenosine triphosphatase activity was unchanged. Bone fat-free weight, fat-free minus ash weight, and bone calcium and phosphorus concentration were reduced significantly in rats given 10 mg/kg Cl2MDP compared to controls. Bone magnesium concentration was significantly elevated in rats given 10 mg/kg Cl2MDP. Serum calcium and phosphorus concentration were lower in Cl2MDP-treated rats. These results suggest that Cl2MDP is capable of altering bone remodeling, enzyme activity and mineral content, without significantly altering bone cell morphology, independent of the effects of parathyroid hormone, calcitonin, and dietary calcium.

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二膦酸二氯亚甲基对去甲状旁腺大鼠骨形态、酶活性及灰分含量的影响。

以4 mg/kg和10 mg/kg的剂量给去甲状旁腺成体大鼠低钙饲料，连续7 d。cl2mdp治疗大鼠的原发性干骺端小梁数量和长度均高于对照组。Cl2MDP对成骨细胞、骨细胞和破骨细胞的光镜和电镜形态无明显影响。Cl2MDP使骨碱性磷酸酶显著升高，而腺苷三磷酸酶活性不变。与对照组相比，给予10 mg/kg Cl2MDP的大鼠骨无脂重、无脂减灰分重和骨钙、磷浓度显著降低。给药10 mg/kg Cl2MDP后，骨镁浓度显著升高。cl2mdp处理大鼠血清钙、磷浓度降低。这些结果表明，Cl2MDP能够改变骨重塑、酶活性和矿物质含量，而不显著改变骨细胞形态，不受甲状旁腺激素、降钙素和膳食钙的影响。

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Calcified Tissue Research

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