[Antibody proteomics].

Q3 Biochemistry, Genetics and Molecular Biology
L Sh Kazieva, T E Farafonova, V G Zgoda
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引用次数: 0

Abstract

Antibodies represent an essential component of humoral immunity; therefore their study is important for molecular biology and medicine. The unique property of antibodies to specifically recognize and bind a certain molecular target (an antigen) determines their widespread application in treatment and diagnostics of diseases, as well as in laboratory and biotechnological practices. High specificity and affinity of antibodies is determined by the presence of primary structure variable regions, which are not encoded in the human genome and are unique for each antibody-producing B cell clone. Hence, there is little or no information about amino acid sequences of the variable regions in the databases. This differs identification of antibody primary structure from most of the proteomic studies because it requires either B cell genome sequencing or de novo amino acid sequencing of the antibody. The present review demonstrates some examples of proteomic and proteogenomic approaches and the methodological arsenal that proteomics can offer for studying antibodies, in particular, for identification of primary structure, evaluation of posttranslational modifications and application of bioinformatics tools for their decoding.

(抗体蛋白质组学)。
抗体是体液免疫的重要组成部分;因此,它们的研究对分子生物学和医学具有重要意义。抗体特异性识别和结合某种分子靶标(抗原)的独特特性决定了它们在疾病的治疗和诊断以及在实验室和生物技术实践中的广泛应用。抗体的高特异性和亲和力是由初级结构可变区域的存在决定的,这些区域不在人类基因组中编码,并且对于每个产生抗体的B细胞克隆都是独特的。因此,数据库中很少或没有关于可变区氨基酸序列的信息。这与大多数蛋白质组学研究的抗体一级结构鉴定不同,因为它需要B细胞基因组测序或抗体的从头氨基酸测序。本综述展示了一些蛋白质组学和蛋白质基因组学方法的例子,以及蛋白质组学可以为研究抗体提供的方法库,特别是用于鉴定初级结构,评估翻译后修饰和应用生物信息学工具进行解码。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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