MiR-200c regulates invasion, proliferation and EMT of anaplastic thyroid cancer cells by targeting parathyroid hormone like hormone.

IF 1.8 4区 生物学 Q4 CELL BIOLOGY
Yan Zhang, Yuanyuan Duan, Chenguang Wu, Wen Peng, Wenyu Chen, Li Wang, Zhaoqun Deng
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引用次数: 0

Abstract

This study aimed to explore the specific effect of miR-200c in anaplastic thyroid cancer (ATC). Hth74 and ARO cell lines were used. Proliferation, invasion, and colony formation activities of Hth74 and ARO cell lines affected by miR-200c were studied. Expression of epithelial-to-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, Slug, and Snail) in the Hth74 and ARO cell lines were validated by western blot and qRT-PCR. In addition, the regulation of the parathyroid hormone-like hormone (PTHLH) by miR-200c was assessed. Overexpression of miR-200c inhibited the invasion, proliferation, and colony formation of the ATC cell lines, whereas its downregulation achieved the opposite results. PTHLH was found to be regulated negatively by miR-200c through a miR-200c binding site within the 3'-UTR of PTHLH. miR-200c repressed the proliferation, invasion, and EMT process of cells in ATC cell lines by targeting PTHLH post-transcriptionally, which indicates that miR-200c may be a potential target for the treatment of ATC.

MiR-200c通过靶向甲状旁腺激素样激素调控间变性甲状腺癌细胞的侵袭、增殖和EMT。
本研究旨在探讨miR-200c在间变性甲状腺癌(ATC)中的特异性作用。采用Hth74和ARO细胞系。研究了miR-200c对Hth74和ARO细胞系的增殖、侵袭和集落形成活性的影响。western blot和qRT-PCR验证了Hth74和ARO细胞系上皮-间质转化(EMT)标志物(E-cadherin、N-cadherin、Slug和Snail)的表达。此外,评估miR-200c对甲状旁腺激素样激素(PTHLH)的调节作用。过表达miR-200c可抑制ATC细胞系的侵袭、增殖和集落形成,而下调miR-200c可达到相反的结果。发现miR-200c通过PTHLH的3'-UTR内的miR-200c结合位点对PTHLH进行负调控。miR-200c通过转录后靶向PTHLH抑制ATC细胞系中细胞的增殖、侵袭和EMT过程,这表明miR-200c可能是治疗ATC的潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Growth factors
Growth factors 生物-内分泌学与代谢
CiteScore
2.60
自引率
0.00%
发文量
20
审稿时长
>12 weeks
期刊介绍: Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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