Evaluation of anti-adenoviral effects of the polyvinyl alcohol iodine ophthalmic solution.

IF 2.1 3区医学 Q2 OPHTHALMOLOGY

Japanese Journal of Ophthalmology Pub Date : 2024-01-01 Epub Date: 2023-11-15 DOI:10.1007/s10384-023-01027-1

Tomoko Tsukahara-Kawamura, Nozomu Hanaoka, Eiichi Uchio

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引用次数: 0

Abstract

Purpose: To investigate the virucidal effects of a polyvinyl alcohol iodine, Saniode, against 16 types of human mastadenovirus (HAdV) causing ophthalmic, respiratory, gastrointestinal, urinary, and systemic infections.

Study design: Laboratory investigation METHODS: Fifty microliters of Saniode were exposed to 10 μL each containing HAdV virus stock solution of 1 × 10⁶ copies/μL of HAdV-1, -2, -3, -4, 5, -6, -7, -8, -11, -37, -53, -54, -56, -64, -81, and -85 for 10 s, 30 s, 1 min, and 3 min. After neutralization with 0.5% sodium thiosulfate, the mixture was diluted by ten-fold serial dilution and inoculated into 24 wells containing confluent A549 cell monolayers. Virucidal effects were calculated relative to the positive control on days 7-10 and observed until 30 days post-infection.

Results: Saniode satisfied the EN-14476 criterion for virucidal effects (>99.99%) for all HAdV types at all exposure times, including at 10 s on days 7 to 10 post-infection. All types of HAdVs that reacted for > 1 min achieved 99.99% reduction, including after 30 days.

Conclusion: Saniode displayed virucidal effects against all tested HAdV types. Currently, with no specific medication available for HAdVs in ocular infection, this could be an option to prevent the spread of keratoconjunctivitis.

Abstract Image

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聚乙烯醇碘眼液抗腺病毒作用的评价。

目的:研究聚乙烯醇碘(Saniode)对16种引起眼部、呼吸道、胃肠道、泌尿和全身感染的人乳腺病毒(hav)的抗病毒作用。方法:将50微升的Saniode分别暴露于含有1 × 106拷贝/μL hav -1、-2、-3、-4、5、-6、-7、-8、-11、-37、-53、-54、-56、-64、-81、-85的hav病毒原液中10 μL、30 s、1 min、3 min，用0.5%硫代硫酸钠中和后，经10倍连续稀释后接种于含有A549细胞单层的24孔中。在感染后7-10天计算相对于阳性对照的杀病毒效果，并观察至感染后30天。结果:Saniode在所有暴露时间(包括感染后第7 ~ 10天的10s)对所有hav类型的毒力均达到EN-14476标准(>99.99%)。所有类型的HAdVs在反应bbb10min后均达到99.99%的还原，包括30天后。结论:三氯乙酸对所有类型的hav均有抗病毒作用。目前，由于没有针对HAdVs眼部感染的特异性药物，这可能是防止角膜结膜炎扩散的一种选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Japanese Journal of Ophthalmology 医学-眼科学

CiteScore

4.80

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Japanese Journal of Ophthalmology (JJO) was inaugurated in 1957 as a quarterly journal published in English by the Ophthalmology Department of the University of Tokyo, with the aim of disseminating the achievements of Japanese ophthalmologists worldwide. JJO remains the only Japanese ophthalmology journal published in English. In 1997, the Japanese Ophthalmological Society assumed the responsibility for publishing the Japanese Journal of Ophthalmology as its official English-language publication. Currently the journal is published bimonthly and accepts papers from authors worldwide. JJO has become an international interdisciplinary forum for the publication of basic science and clinical research papers.