CircUsp9x/miR-599/stim1 axis regulates proliferation and migration in vascular smooth muscle cells induced by oxidized-low density lipoprotein.

IF 1.5 4区医学 Q3 PERIPHERAL VASCULAR DISEASE

Clinical and Experimental Hypertension Pub Date : 2023-12-31 Epub Date: 2023-11-14 DOI:10.1080/10641963.2023.2280758

Hui-Lin Hu, Hui-Xiu Zheng, Na Yuan, Chang-Lin Zhai, Hao Chen, Hai-Hua Pan, Gang Qian

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引用次数: 0

Abstract

Circular RNAs (circRNAs) regulate the function of vascular smooth muscle cells (VSMCs) in atherosclerosis (AS) progression. We aimed to explore the role of circUSP9X in oxidized low-density lipoprotein (ox-LDL)-induced VSMCs. Cell proliferation was assessed using cell counting kit-8 and EDU assays. Cell migration was evaluated using Transwell and wound healing assays. The interaction between circUSP9X or STIM1 and miR-599 was analyzed using dual-luciferase reporter and RNA pull-down assays. Their levels were examined using quantitative real-time PCR. CircUSP9X and STIM1 expression was increased, whereas miR-599 expression was reduced in the serum of patients with AS and ox-LDL-stimulated VSMCs. Overexpression of circUSP9X facilitated the proliferation and migration of VSMCs induced by ox-LDL. CircUSP9X sponged miR-599, which targeted STIM1. MiR-599 reversed the effects induced by circUSP9X, and STIM1 reversed the effects induced by miR-599. Taken together, CircUSP9X promoted proliferation and migration in ox-LDL-treated VSMCs via the miR-599/STIM1 axis, providing a theoretical basis for the role of circUSP9X/miR-599/STIM1 axis in AS.

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CircUsp9x/miR-599/stim1轴调控氧化低密度脂蛋白诱导的血管平滑肌细胞的增殖和迁移。

环状rna (circRNAs)在动脉粥样硬化(AS)进展中调节血管平滑肌细胞(VSMCs)的功能。我们旨在探讨circUSP9X在氧化低密度脂蛋白(ox-LDL)诱导的VSMCs中的作用。采用细胞计数试剂盒-8和EDU测定细胞增殖。采用Transwell法和伤口愈合法评估细胞迁移。使用双荧光素酶报告基因和RNA下拉实验分析circUSP9X或STIM1与miR-599之间的相互作用。采用实时荧光定量PCR检测其水平。AS和ox- ldl刺激的VSMCs患者血清中CircUSP9X和STIM1表达升高，而miR-599表达降低。circUSP9X的过表达促进ox-LDL诱导的VSMCs的增殖和迁移。CircUSP9X擦拭了靶向STIM1的miR-599。MiR-599逆转了circUSP9X诱导的作用，STIM1逆转了MiR-599诱导的作用。综上所述，CircUSP9X通过miR-599/STIM1轴促进ox- ldl处理的vsmc的增殖和迁移，为CircUSP9X /miR-599/STIM1轴在AS中的作用提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical and Experimental Hypertension 医学-外周血管病

CiteScore

3.90

自引率

0.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Clinical and Experimental Hypertension is a reputable journal that has converted to a full Open Access format starting from Volume 45 in 2023. While previous volumes are still accessible through a Pay to Read model, the journal now provides free and open access to its content. It serves as an international platform for the exchange of up-to-date scientific and clinical information concerning both human and animal hypertension. The journal publishes a wide range of articles, including full research papers, solicited and unsolicited reviews, and commentaries. Through these publications, the journal aims to enhance current understanding and support the timely detection, management, control, and prevention of hypertension-related conditions. One notable aspect of Clinical and Experimental Hypertension is its coverage of special issues that focus on the proceedings of symposia dedicated to hypertension research. This feature allows researchers and clinicians to delve deeper into the latest advancements in this field. The journal is abstracted and indexed in several renowned databases, including Pharmacoeconomics and Outcomes News (Online), Reactions Weekly (Online), CABI, EBSCOhost, Elsevier BV, International Atomic Energy Agency, and the National Library of Medicine, among others. These affiliations ensure that the journal's content receives broad visibility and facilitates its discoverability by professionals and researchers in related disciplines.