Engineered Cas13 variants with minimal collateral RNA targeting

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2022-08-12 DOI:10.1038/s41587-022-01423-x

引用次数: 0

Abstract

The application of CRISPR–Cas13 for targeted RNA degradation is hindered by the existence of collateral effects induced by Cas13. We screened hundreds of engineered Cas13 variants using a convenient and sensitive reporter system and obtained several variants with markedly reduced collateral activity and efficient on-target activity, suitable for in vivo applications.

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具有最小附带 RNA 靶向性的工程 Cas13 变体

CRISPR-Cas13在靶向降解RNA方面的应用因Cas13诱导的附带效应的存在而受到阻碍。我们利用一种方便灵敏的报告系统筛选了数百种Cas13工程变体，获得了几种附带活性明显降低、靶上活性高效的变体，适合体内应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

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