Ultrastructural changes induced by açaí (Euterpe oleracea Mart) in MCF-7 breast cancer cell line.

IF 1.1 4区医学 Q4 MICROSCOPY

Ultrastructural Pathology Pub Date : 2022-11-02 DOI:10.1080/01913123.2022.2141404

Marcos Antonio Custódio Neto da Silva, Camila Simões Soares, Kátia Regina Assunção Borges, Laís Araujo Souza Wolff, Maria Do Carmo Lacerda Barbosa, Maria Do Desterro Soares Brandão Nascimento, João Ernesto de Carvalho

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引用次数: 1

Abstract

Açaí (Euterpe oleracea Mart) is an Amazon plant with many biological properties. Previous report of this group evidenced autophagy induction after treatment with açaí seed extract in MCF-7 breast cancer cell lines by acridine orange assay. The aim of this study was to evaluate the ultrastructural changes induced by açaí seed extract in MCF-7 breast cancer cell lines. First, MCF- 7 breast cancer cell line viability was evaluated by MTT assay. Acridine orange assay showed increase in the acidic compartments, suggesting autophagolysosome formation. These cells were treated with 25 μg/ml for 24 h and evaluated by transmission electron microscopy (MET). This analysis showed that açaí seed extract induced autophagy, confirmed by autophagolysosome formation. Furthermore, açaí seed extract increased the number of mitochondria, suggesting the enrollment of reactive oxygen species in autophagy.

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açaí (Euterpe oleracea Mart)诱导MCF-7乳腺癌细胞系超微结构改变。

Açaí (Euterpe oleracea Mart)是一种亚马逊植物，具有许多生物学特性。该组先前的报道通过吖啶橙试验证实了açaí种子提取物在MCF-7乳腺癌细胞株中诱导自噬。研究açaí种子提取物对MCF-7乳腺癌细胞株超微结构的影响。首先，采用MTT法评价MCF- 7乳腺癌细胞系的生存能力。吖啶橙试验显示酸性区室增加，提示自噬溶酶体形成。25 μg/ml处理24 h，透射电镜观察。分析表明açaí种子提取物诱导自噬，自噬溶酶体的形成证实了这一点。此外，açaí种子提取物增加了线粒体的数量，表明活性氧参与了自噬。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ultrastructural Pathology 医学-病理学

CiteScore

2.00

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ultrastructural Pathology is the official journal of the Society for Ultrastructural Pathology. Published bimonthly, we are the only journal to be devoted entirely to diagnostic ultrastructural pathology. Ultrastructural Pathology is the ideal journal to publish high-quality research on the following topics: Advances in the uses of electron microscopic and immunohistochemical techniques Correlations of ultrastructural data with light microscopy, histochemistry, immunohistochemistry, biochemistry, cell and tissue culturing, and electron probe analysis Important new, investigative, clinical, and diagnostic EM methods.