Karyotype and COI gene sequences of Chironomusmelanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems.

IF 1 4区生物学 Q4 GENETICS & HEREDITY

Comparative Cytogenetics Pub Date : 2022-01-01 DOI:10.3897/compcytogen.v16.i3.90336

Viktor V Bolshakov, Ekaterina A Movergoz

{"title":"Karyotype and COI gene sequences of Chironomusmelanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems.","authors":"Viktor V Bolshakov, Ekaterina A Movergoz","doi":"10.3897/compcytogen.v16.i3.90336","DOIUrl":null,"url":null,"abstract":"Karyotype and COI gene sequences of Chironomusmelanotus Keyl, 1961 from the Yaroslavl region (Russia) were analyzed. A low level of chromosomal polymorphism has been confirmed, eventually eight banding sequences were found: melA1, melB1, melC1, melD1, melE1, melF1, and melG1; only melD2 was found in two larvae from the Sunoga river. Analysis of phylogenetic tree and estimated genetic distances has shown not all COI gene sequences of Ch.melanotus in GenBank and BOLD to belong to this species. The lower distance of 0.4% was observed between two sequences from the Yaroslavl region and Finland, apparently these are true Ch.melanotus sequences. The distances between true Ch.melanotus and other sequences from Finland were 9.5% and 12.4%, and from Sweden it was 11%. The average genetic distance between studied sequences of 9.1% is out of the range of the 3% threshold previously determined for chironomids. According to our estimates, there are two sequences with a distance of 2.9% that may belong to Ch.annularius Meigen, 1818, and one sequence with a genetic distance of 2.1%, may belonging to Ch.cingulatus Meigen, 1830, which has been confirmed karyologically. Another two sequences form a separate cluster. We suggest that they either belong to a known species, but are not present in the databases, or belong to a distinct, undescribed species.","PeriodicalId":50656,"journal":{"name":"Comparative Cytogenetics","volume":"16 3","pages":"161-172"},"PeriodicalIF":1.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9849057/pdf/","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Cytogenetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3897/compcytogen.v16.i3.90336","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 3

Abstract

Karyotype and COI gene sequences of Chironomusmelanotus Keyl, 1961 from the Yaroslavl region (Russia) were analyzed. A low level of chromosomal polymorphism has been confirmed, eventually eight banding sequences were found: melA1, melB1, melC1, melD1, melE1, melF1, and melG1; only melD2 was found in two larvae from the Sunoga river. Analysis of phylogenetic tree and estimated genetic distances has shown not all COI gene sequences of Ch.melanotus in GenBank and BOLD to belong to this species. The lower distance of 0.4% was observed between two sequences from the Yaroslavl region and Finland, apparently these are true Ch.melanotus sequences. The distances between true Ch.melanotus and other sequences from Finland were 9.5% and 12.4%, and from Sweden it was 11%. The average genetic distance between studied sequences of 9.1% is out of the range of the 3% threshold previously determined for chironomids. According to our estimates, there are two sequences with a distance of 2.9% that may belong to Ch.annularius Meigen, 1818, and one sequence with a genetic distance of 2.1%, may belonging to Ch.cingulatus Meigen, 1830, which has been confirmed karyologically. Another two sequences form a separate cluster. We suggest that they either belong to a known species, but are not present in the databases, or belong to a distinct, undescribed species.

Abstract Image

查看原文本刊更多论文

俄罗斯雅罗斯拉夫尔地区1961年Chironomusmelanotus Keyl的Karyotype和COI基因序列及其GenBank和BOLD系统鉴定的困难

对俄罗斯雅罗斯拉夫尔(Yaroslavl)地区1961种Chironomusmelanotus Keyl的核型和COI基因序列进行了分析。证实了低水平的染色体多态性，最终发现了八个带序列:melA1, melB1, melC1, melD1, melE1, melF1和melG1;只有melD2在两条来自Sunoga河的幼虫中被发现。系统发育树分析和估计遗传距离表明，GenBank和BOLD中黑毛鼠的COI基因序列并非都属于该物种。来自雅罗斯拉夫尔地区和芬兰的两个序列之间的距离较低，为0.4%，显然这是真实的黑斑切鼠序列。芬兰和瑞典的真黑斑切蚊与其他序列的距离分别为9.5%和12.4%，瑞典为11%。所研究序列之间的平均遗传距离为9.1%，超出了先前确定的摇尾鱼3%的阈值范围。根据我们的估计，有两个序列的遗传距离为2.9%，可能属于1818年的Ch.annularius Meigen，一个序列的遗传距离为2.1%，可能属于1830年的Ch.cingulatus Meigen，这已经得到了核学上的证实。另外两个序列形成一个单独的簇。我们认为它们要么属于一个已知的物种，但没有出现在数据库中，要么属于一个独特的，未被描述的物种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Comparative Cytogenetics 生物-遗传学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Comparative Cytogenetics is a peer-reviewed, open-access, rapid online journal launched to accelerate research on all aspects of plant and animal cytogenetics, karyosystematics, and molecular systematics. All published papers can be freely copied, downloaded, printed and distributed at no charge for the reader. Authors are thus encouraged to post the pdf files of published papers on their homepages or elsewhere to expedite distribution. There is no charge for color.

﻿Karyotype and COI gene sequences of Chironomusmelanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems.

Abstract

Karyotype and COI gene sequences of Chironomusmelanotus Keyl, 1961 from the Yaroslavl region, Russia, and the difficulties with its identification using GenBank and BOLD systems.