Karyotype description and comparative chromosomal mapping of rDNA and U2 snDNA sequences in Eigenmannialimbata and E.microstoma (Teleostei, Gymnotiformes, Sternopygidae).
Cristian Andrés Araya-Jaime, Duílio Mazzoni Zerbinato de Andrade Silva, Luís Ricardo Ribeiro da Silva, Cristiano Neves do Nascimento, Claudio Oliveira, Fausto Foresti
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引用次数: 1
Abstract
The genus Eigenmannia Jordan et Evermann,1896 includes electric fishes endemic to the Neotropical region with extensive karyotype variability and occurrence of different sex chromosome systems, however, cytogenetic studies within this group are restricted to few species. Here, we describe the karyotypes of Eigenmannialimbata (Schreiner et Miranda Ribeiro, 1903) and E.microstoma (Reinhardt, 1852) and the chromosomal locations of 5S and 18S rDNAs (ribosomal RNA genes) and U2 snDNA (small nuclear RNA gene). Among them, 18S rDNA sites were situated in only one chromosomal pair in both species, and co-localized with 5S rDNA in E.microstoma. On the other hand, 5S rDNA and U2 snRNA sites were observed on several chromosomes, with variation in the number of sites between species under study. These two repetitive DNAs were observed co-localized in one chromosomal pair in E.limbata and in four pairs in E.microstoma. Our study shows a new case of association of these two types of repetitive DNA in the genome of Gymnotiformes.
Eigenmannia Jordan et Evermann,1896包括新热带地区特有的电鱼，具有广泛的核型变异和不同性染色体系统的发生，然而，对这一群体的细胞遗传学研究仅限于少数物种。本文描述了Eigenmannialimbata (Schreiner et Miranda Ribeiro, 1903)和E.microstoma (Reinhardt, 1852)的核型，以及5S和18S rnas(核糖体RNA基因)和U2 snDNA(小核RNA基因)的染色体位置。其中，18S rDNA位点在两种物种中仅位于一对染色体上，并且在小口棘球绦虫中与5S rDNA共定位。另一方面，在一些染色体上观察到5S rDNA和U2 snRNA位点，在研究的物种之间，位点的数量存在差异。这两种重复的dna分别位于limbata的1对染色体上和microstoma的4对染色体上。我们的研究显示了这两种类型的重复DNA在裸子形动物基因组中的关联的新情况。
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