Association of GCK (rs1799884), GCKR (rs780094), and G6PC2 (rs560887) Gene Polymorphisms with Type 2 Diabetes among Malay Ethnics.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.1055/s-0042-1760384

Neda Ansari, Vasudevan Ramachandran, Nur Afiqah Mohamad, Elnaz Salim, Patimah Ismail, Mohamad Hazmi, Liyana Najwa Inchee Mat

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引用次数: 1

Abstract

Background Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder, and the underlying causes remain unknown and have not been fully elucidated. Several candidate genes have been associated with T2DM in various populations with conflicting results. The variations found in glucokinase ( GCK ), glucokinase regulatory protein ( GCKR ), and glucose-6-phosphatase 2 ( G6PC2 ) genes were not well studied, particularly among Asians. Aims The main objective of this study was to determine the candidate genetic polymorphisms of GCK (rs1799884), GCKR (rs780094), and G6PC2 (rs560887) genes in T2DM among Malay ethnics. Methods In this candidate gene association study, a total of 180 T2DM subjects and 180 control subjects were recruited to determine the genotypes using polymerase chain reaction-restriction fragment length polymorphism and Taqman probe assay methods. Genotype and allele frequencies in case and control samples were compared using the chi-squared test to determine a significant difference. Results The body mass index, fasting blood glucose, hemoglobin A1c, systolic and diastolic blood pressure, and total cholesterol were significantly different ( p < 0.05) between T2DM and control subjects. The genotypic and allelic frequencies of GCK (rs1799884), GCKR (rs780094), and G6PC2 (rs560887) gene polymorphisms were significantly different between T2DM and controls ( p < 0.05). Conclusion Hence, rs1799884 of GCK gene and rs780094 of GCKR gene and rs560887 of the G6PC2 gene are possible genetic biomarkers in T2DM development among Malay ethnics in Malaysia.

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马来人GCK (rs1799884)、GCKR (rs780094)和G6PC2 (rss560887)基因多态性与2型糖尿病的关系

背景2型糖尿病(T2DM)是一种复杂的代谢性疾病，其根本原因尚不清楚，也尚未完全阐明。几个候选基因与不同人群的T2DM相关，但结果相互矛盾。在葡萄糖激酶(GCK)、葡萄糖激酶调节蛋白(GCKR)和葡萄糖-6-磷酸酶2 (G6PC2)基因中发现的变异尚未得到很好的研究，特别是在亚洲人中。本研究的主要目的是确定GCK (rs1799884)、GCKR (rs780094)和G6PC2 (rs560887)基因在马来民族T2DM中的候选遗传多态性。方法采用聚合酶链反应-限制性片段长度多态性和Taqman探针法，对180例T2DM患者和180例对照组进行基因型分析。病例和对照样本的基因型和等位基因频率采用卡方检验比较，以确定显著差异。结果T2DM患者体质量指数、空腹血糖、血红蛋白A1c、收缩压、舒张压、总胆固醇差异有统计学意义(p GCK (rs1799884)、GCKR (rs780094)、G6PC2 (rs560887)基因多态性差异有统计学意义(p结论GCK基因rs1799884、GCKR基因rs780094、G6PC2基因rss560887可能是马来西亚马来族T2DM发病的遗传生物标志物)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.