Spurious Serum Hormone Immunoassay Results: Causes, Recognition, Management.

Glenn D Braunstein
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引用次数: 2

Abstract

For over 50 years, immunoassays have been extensively used to quantitate hormones in blood, other fluids and tissues. Each assay has its own sensitivity, specificity and other analytical components. Despite the differences between commercial products, these assays provide important clinical information about hormone levels in patients. However, inaccurate results can occur because of technical issues, as well as patient-specific factors that can interfere with immunoassay hormone measurements. The latter include excessive normal blood or serum components, the presence of cross-reacting substances, extremely high levels of hormones leading to the high-dose hook effect, and interference from a variety of endogenous factors such as human antibodies that interact with the assay components or high levels of biotin in the serum from exogenous ingestion. This article briefly reviews the sources and recognition of endogenous interference, and describes methods to determine the correct serum hormone concentration.

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假血清激素免疫分析结果:原因,识别,处理。
50多年来,免疫测定法被广泛用于定量血液、其他液体和组织中的激素。每种分析方法都有自己的敏感性、特异性和其他分析成分。尽管商业产品之间存在差异,但这些检测提供了关于患者激素水平的重要临床信息。然而,由于技术问题以及可能干扰免疫测定激素测量的患者特异性因素,结果可能不准确。后者包括过量的正常血液或血清成分,交叉反应物质的存在,导致高剂量钩效应的极高水平的激素,以及各种内源性因素的干扰,如与测定成分相互作用的人抗体或外源性摄入的血清中高水平的生物素。本文简要综述了内源性干扰的来源和识别方法,并介绍了正确测定血清激素浓度的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
2.40
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