Orchestrating recombination initiation in mice and men.

2区生物学 Q1 Biochemistry, Genetics and Molecular Biology

Current Topics in Developmental Biology Pub Date : 2023-01-01 Epub Date: 2022-08-08 DOI:10.1016/bs.ctdb.2022.05.001

Elena Damm, Linda Odenthal-Hesse

{"title":"Orchestrating recombination initiation in mice and men.","authors":"Elena Damm, Linda Odenthal-Hesse","doi":"10.1016/bs.ctdb.2022.05.001","DOIUrl":null,"url":null,"abstract":"<p><p>Recent discoveries have advanced our understanding of recombination initiation beyond the placement of double-stranded DNA breaks (DSBs) from germline replication timing to the dynamic reorganization of chromatin, and defined critical players of recombination initiation. This article focuses on recombination initiation in mammals utilizing the PRDM9 protein to orchestrate crucial stages of meiotic recombination initiation by interacting with the local DNA environment and several protein complexes. The Pioneer Complex with the SNF2-type chromatin remodeling enzyme HELLS, exposes PRDM9-bound DNA. At the same time, a Compass-Complex containing EWSR1, CXXC1, CDYL, EHMT2 and PRDM9 facilitates the association of putative hotspot sites in DNA loops with the chromosomal axis where DSB-promoting complexes are located, and DSBs are catalyzed by the SPO11/TOPOVIBL complex. Finally, homology search is facilitated at PRDM9-directed sites by ANKRD31. The Reader-Writer system consists of PRDM9 writing characteristic histone methylation signatures, which are read by ZCWPW1, promoting efficient homology engagement.</p>","PeriodicalId":55191,"journal":{"name":"Current Topics in Developmental Biology","volume":"151 ","pages":"27-42"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Topics in Developmental Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/bs.ctdb.2022.05.001","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/8/8 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 0

Abstract

Recent discoveries have advanced our understanding of recombination initiation beyond the placement of double-stranded DNA breaks (DSBs) from germline replication timing to the dynamic reorganization of chromatin, and defined critical players of recombination initiation. This article focuses on recombination initiation in mammals utilizing the PRDM9 protein to orchestrate crucial stages of meiotic recombination initiation by interacting with the local DNA environment and several protein complexes. The Pioneer Complex with the SNF2-type chromatin remodeling enzyme HELLS, exposes PRDM9-bound DNA. At the same time, a Compass-Complex containing EWSR1, CXXC1, CDYL, EHMT2 and PRDM9 facilitates the association of putative hotspot sites in DNA loops with the chromosomal axis where DSB-promoting complexes are located, and DSBs are catalyzed by the SPO11/TOPOVIBL complex. Finally, homology search is facilitated at PRDM9-directed sites by ANKRD31. The Reader-Writer system consists of PRDM9 writing characteristic histone methylation signatures, which are read by ZCWPW1, promoting efficient homology engagement.

查看原文本刊更多论文

协调小鼠和人类的重组启动

最近的发现推进了我们对重组启动的理解，从种系复制时序的双链DNA断裂（DSB）位置到染色质的动态重组，并确定了重组启动的关键参与者。本文重点研究哺乳动物的重组启动，利用 PRDM9 蛋白与局部 DNA 环境和几个蛋白复合物相互作用，协调减数分裂重组启动的关键阶段。带有 SNF2 型染色质重塑酶 HELLS 的先锋复合体暴露了与 PRDM9 结合的 DNA。与此同时，包含 EWSR1、CXXC1、CDYL、EHMT2 和 PRDM9 的 Compass-Complex 促进了 DNA 环中的推定热点位点与染色体轴的结合，而染色体轴上有促进 DSB 的复合物，DSB 由 SPO11/TOPOVIBL 复合物催化。最后，ANKRD31 在 PRDM9 指向的位点促进同源性搜索。阅读器-书写器系统包括 PRDM9 书写特征组蛋白甲基化签名，ZCWPW1 阅读这些签名，从而促进有效的同源啮合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Topics in Developmental Biology 生物-发育生物学

CiteScore

6.00

自引率

0.00%

发文量