Evaluation of MUC1, MUC2, MUC5AC, and MUC6 Expression Differences in Lung Adenocarcinoma Subtypes by Using a Final Immunoreactivity Score (FIRS).

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-01-01 DOI:10.5146/tjpath.2022.01593

Melek Buyuk, Yasemin Ozluk, Dogu Vuralli Bakkaloglu, Berker Ozkan, Pinar Firat, Dilek Yilmazbayhan

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Abstract

Objective: Lung adenocarcinomas are divided into acinar, lepidic, papillary, micropapillary, and solid predominant subtypes according to the current World Health Organization (WHO) classification. We designed this retrospective study to demonstrate profiles of MUC expression (MUC1, MUC2, MUC5AC, and MUC6) of different histologic patterns within the same tumor among pulmonary adenocarcinomas and investigate correlations of MUC expression with clinicopathologic features.

Material and method: We analyzed the expression of mucins (MUC1, MUC2, MUC5AC, and MUC6) in a series of 99 resected lung adenocarcinomas, which included a total of 193 patterns (71 acinar, 30 lepidic, 25 papillary, 20 micropapillary, 34 solid and 13 mucinous) and calculated a final immune reactivity score (FIRS) per tumor.

Results: MUC1 IRS scores were significantly higher in lepidic and solid patterns compared with mucinous patterns (p=0.013). MUC2 expression was seen only in three cases (1 acinar, 2 mucinous). MUC5AC and MUC2 expression was more common in mucinous patterns (p < 0.001 and p=0.028, respectively). MUC6 expression was only detected in seven patterns and the expression was weak. No significant difference was seen among histologic patterns for the staining scores of MUC6. Mucinous adenocarcinoma differed from other histologic subtypes regarding MUC1 and MUC5AC expression. Mucinous adenocarcinoma showed less MUC1 expression with lower IRS scores and higher MUC5AC expression. Tumor size (p=0.006), lymphatic invasion (p=0.018), vascular invasion (p=0.025), perineural invasion (p=0.019), MUC1 IRS scores (p=0.018), and MUC1 IRS scores > 8.5 (p=0.018) were significant predictors for lymph node metastasis.

Conclusion: An alternative scoring for MUC1 can be used as a predictor for lymph node metastasis regardless of the histologic subtype.

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通过使用最终免疫反应性评分（FIRS）评估肺腺癌亚型中MUC1、MUC2、MUC5AC和MUC6表达差异。

目的：根据世界卫生组织（世界卫生组织）的分类，肺腺癌可分为腺泡型、鳞泡型、乳头型、微乳头型和实体型。我们设计了这项回顾性研究，以证明肺腺癌中同一肿瘤内不同组织学模式的MUC表达谱（MUC1、MUC2、MUC5AC和MUC6），并研究MUC表达与临床病理特征的相关性。材料和方法：我们分析了粘蛋白（MUC1、MUC2、MUC5AC和MUC6）在一系列99例切除的肺腺癌中的表达，其中包括总共193种模式（71种腺泡型、30种鳞翅型、25种乳头型、20种微乳头型、34种实体型和13种粘蛋白型），并计算了每个肿瘤的最终免疫反应性评分（FIRS）。结果：与粘液型相比，鳞状和实体型的MUC1 IRS评分显著较高（p=0.013）。MUC2仅在3例（1例腺泡，2例粘液）中表达。MUC5AC和MUC2的表达在粘液性模式中更常见（分别为p＜0.001和p＝0.028）。MUC6的表达仅在7种模式中检测到，并且表达较弱。MUC6的染色评分在组织学模式之间没有显著差异。粘液腺癌在MUC1和MUC5AC表达方面与其他组织学亚型不同。粘液腺癌表现出较少的MUC1表达，具有较低的IRS评分和较高的MUC5AC表达。肿瘤大小（p=0.006）、淋巴浸润（p=0.018）、血管浸润（p=0.025）、神经周浸润（p=0.019）、MUC1-IRS评分（p=0.008）和MUC1-IRS评分>8.5（p=0.018）是淋巴结转移的重要预测因素。结论：无论组织学亚型如何，MUC1的替代评分都可以作为淋巴结转移的预测指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.