Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran.

IF 2.4 Q1 PEDIATRICS
Sahar Sabour, Amir Teimourpour, Jafar Mohammadshahi, Hadi Peeridogaheh, Roghayeh Teimourpour, Taher Azimi, Zahra Hosseinali
{"title":"Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran.","authors":"Sahar Sabour,&nbsp;Amir Teimourpour,&nbsp;Jafar Mohammadshahi,&nbsp;Hadi Peeridogaheh,&nbsp;Roghayeh Teimourpour,&nbsp;Taher Azimi,&nbsp;Zahra Hosseinali","doi":"10.1186/s40348-022-00152-0","DOIUrl":null,"url":null,"abstract":"<p><p>Shigellosis is one of the acute bowel infections and remains a serious public health problem in resource-poor countries. The present study aimed to survey the distribution of extended-spectrum β-lactamase (ESBL)-producing Shigella strains isolated from patients with diarrhea in northwest Iran. In the present cross-sectional study, from January 2019 to December 2020, 1280 fecal samples were collected from children with diarrhea in Ardabil, Iran. Multiplex PCR assay was applied for the presence of ipaH, invC, wbgZ, rfpB, and rfc genes to detect Shigella spp., Shigella sonnei, Shigella dysenteriae, Shigella flexneri, and Shigella boydii, respectively. Phenotypic detection of ESBL-producing isolates was carried out using the Double Disc Test (DDT). The frequency of main ESBL encoding genes including bla<sub>CTX-M</sub>, bla<sub>SHV</sub>, and bla<sub>TEM</sub> was detected using multiplex PCR. The genetic similarity of S. sonnei isolates was determined using ERIC PCR. A total of 49 Shigella isolates (3.8%; 49/1280) including 42 (85.7%) S. sonnei, 5 (10.2%) S. flexneri, and 2 (4%) S. dysenteriae were identified. S. boydii was not detected in any fecal samples. ESBLs were produced by 10.2% of Shigella spp. including 3 S. sonnei, 1 S. flexneri, and 1 S. dysenteriae. The ESBL encoding genes include bla<sub>CTX-M</sub> and bla<sub>TEM</sub> found in 65.3% and 61.2% of isolates, respectively. bla<sub>SHV</sub> gene was not detected in any isolates. The ERIC-PCR profiles allowed the differentiation of 42 S. sonnei strains into 6 clusters. Our study revealed a high frequency of ESBL-encoding genes among Shigella spp. in northwest Iran. The high prevalence of S. sonnei harboring ESBL genes, in the present work, is the main challenge for dysentery treatment, and this concern justifies the need for effective and regular monitoring of antibiotic usage among patients.</p>","PeriodicalId":74215,"journal":{"name":"Molecular and cellular pediatrics","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9732178/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular and cellular pediatrics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1186/s40348-022-00152-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PEDIATRICS","Score":null,"Total":0}
引用次数: 0

Abstract

Shigellosis is one of the acute bowel infections and remains a serious public health problem in resource-poor countries. The present study aimed to survey the distribution of extended-spectrum β-lactamase (ESBL)-producing Shigella strains isolated from patients with diarrhea in northwest Iran. In the present cross-sectional study, from January 2019 to December 2020, 1280 fecal samples were collected from children with diarrhea in Ardabil, Iran. Multiplex PCR assay was applied for the presence of ipaH, invC, wbgZ, rfpB, and rfc genes to detect Shigella spp., Shigella sonnei, Shigella dysenteriae, Shigella flexneri, and Shigella boydii, respectively. Phenotypic detection of ESBL-producing isolates was carried out using the Double Disc Test (DDT). The frequency of main ESBL encoding genes including blaCTX-M, blaSHV, and blaTEM was detected using multiplex PCR. The genetic similarity of S. sonnei isolates was determined using ERIC PCR. A total of 49 Shigella isolates (3.8%; 49/1280) including 42 (85.7%) S. sonnei, 5 (10.2%) S. flexneri, and 2 (4%) S. dysenteriae were identified. S. boydii was not detected in any fecal samples. ESBLs were produced by 10.2% of Shigella spp. including 3 S. sonnei, 1 S. flexneri, and 1 S. dysenteriae. The ESBL encoding genes include blaCTX-M and blaTEM found in 65.3% and 61.2% of isolates, respectively. blaSHV gene was not detected in any isolates. The ERIC-PCR profiles allowed the differentiation of 42 S. sonnei strains into 6 clusters. Our study revealed a high frequency of ESBL-encoding genes among Shigella spp. in northwest Iran. The high prevalence of S. sonnei harboring ESBL genes, in the present work, is the main challenge for dysentery treatment, and this concern justifies the need for effective and regular monitoring of antibiotic usage among patients.

Abstract Image

Abstract Image

Abstract Image

伊朗西北地区儿童腹泻中携带广谱β-内酰胺酶基因的志贺氏菌的分子检测与特征分析。
志贺氏菌病是一种急性肠道感染,在资源贫乏的国家仍然是一个严重的公共卫生问题。本研究旨在调查伊朗西北部腹泻患者中产β-内酰胺酶(ESBL)的志贺氏菌的分布。在本横断面研究中,从2019年1月至2020年12月,从伊朗阿达比尔的腹泻儿童中收集了1280份粪便样本。采用多重PCR法检测ipaH、invC、wbgZ、rfpB和rfc基因的存在,分别检测志贺氏菌、sonneshigella、痢疾志贺氏菌、flexneri志贺氏菌和boydii志贺氏菌。采用双圆盘试验(DDT)对产esbl分离株进行表型检测。采用多重PCR检测ESBL主要编码基因blaCTX-M、blaSHV、blaTEM的频率。采用ERIC PCR技术对sonnei菌株进行遗传相似性分析。共有49株志贺氏菌分离株(3.8%;其中sonnei沙门氏菌42株(85.7%)、flexneri沙门氏菌5株(10.2%)、dysenteriae沙门氏菌2株(4%)。粪便标本中未检出波氏弓形虫。10.2%的志贺氏菌产生ESBLs,包括3株sonnei、1株flexneri和1株痢疾杆菌。ESBL编码基因包括blaCTX-M和blaTEM,分别在65.3%和61.2%的分离株中发现。未检出blaSHV基因。ERIC-PCR图谱可将42株sonnei菌株划分为6个聚类。我们的研究发现,在伊朗西北部的志贺氏菌中,esbl编码基因的频率很高。在目前的工作中,携带ESBL基因的sonnei的高流行率是痢疾治疗的主要挑战,这一担忧证明了对患者抗生素使用进行有效和定期监测的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.20
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信