Effects of isotretinoin and acitretin on neuroregeneration in experimental spinal cord injury.

IF 1.1 4区 医学 Q3 ORTHOPEDICS
Güven Kılıç, Ömer Polat, Doğan Şensoy, Hakan Soylu
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引用次数: 0

Abstract

Objective: This study aimed to determine whether isotretinoin and acitretin have beneficial effects on neural tissue damage following acute spinal cord injury.

Methods: Thirty-six rats were randomly divided into 6 groups: control, sham spinal cord injury, spinal cord injury with isotretinoin 15 mg/kg for 14 days, spinal cord injury with isotretinoin 15 mg/kg for 28 days, spinal cord injury with acitretin 10 mg/kg for 14 days, and spinal cord injury with acitretin 10 mg/kg for 28 days. The damage to the spinal cord was formed by the clip compression technique. A neurological evaluation was conducted on days 1, 14, and 28. All rats were sacrificed following the treatment period, and samples of their spinal cords were collected for histopathological analysis.

Results: The inclined plane angle was significantly increased on the 14th and 28th days in the isotretinoin 15 mg and acitretin 10 mg groups, compared to the spinal injury group (P=.049 and P=.009, respectively). The Drummond-Moore criterion was significantly higher in the acitretin 10 mg group than in the injury group (P=.026). Cleaved Caspase-3 expression was similar in the isotretinoin 15 mg day 28 group and the control group (P > .05), but significantly decreased in the acitretin 10 mg 14th-day and acitretin 10 mg 28th-day groups compared to spinal injury isotretinoin 15 mg 14th-day and isotretinoin 15 mg 28th-day groups (P < .05).

Conclusion: This was the first study elaborating that isotretinoin and acitretin reduced neuronal apoptosis and improved functional recovery after spinal cord injury. These neuroprotective effects might open a window of opportunity for patients.

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异维甲酸和阿片对实验性脊髓损伤神经再生的影响。
目的:本研究旨在确定异维甲酸和阿曲汀是否对急性脊髓损伤后的神经组织损伤有有益作用。方法:将36只大鼠随机分为6组:对照组、假脊髓损伤组、异维甲酸15mg/kg脊髓损伤14d组、异维菌素15mg/kg脊髓创伤28d组、阿曲素10mg/kg脊髓损伤14dd组和阿曲素10mg/kg脊髓损伤28d组。脊髓损伤是通过夹压技术形成的。在第1、14和28天进行神经学评估。治疗期结束后处死所有大鼠,并收集其脊髓样本进行组织病理学分析。结果:与脊髓损伤组相比,异维甲酸15 mg组和阿曲汀10 mg组的倾斜平面角在第14天和第28天显著增加(分别为P=0.049和P=0.009)。阿曲汀10mg组的Drummond-Moore标准显著高于损伤组(P=0.026)。异维甲酸15mg第28天组和对照组的裂解半胱天冬酶-3表达相似(P>0.05),与脊髓损伤后异维甲酸15mg第14天和异维甲酸15mg第28天组相比,阿曲素10mg第14天组和阿曲素10 mg第28天组显著降低(P<0.05)。这些神经保护作用可能为患者打开机会之窗。
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来源期刊
CiteScore
2.00
自引率
0.00%
发文量
66
审稿时长
>12 weeks
期刊介绍: Acta Orthopaedica et Traumatologica Turcica (AOTT) is an international, scientific, open access periodical published in accordance with independent, unbiased, and double-blinded peer-review principles. The journal is the official publication of the Turkish Association of Orthopaedics and Traumatology, and Turkish Society of Orthopaedics and Traumatology. It is published bimonthly in January, March, May, July, September, and November. The publication language of the journal is English. The aim of the journal is to publish original studies of the highest scientific and clinical value in orthopedics, traumatology, and related disciplines. The scope of the journal includes but not limited to diagnostic, treatment, and prevention methods related to orthopedics and traumatology. Acta Orthopaedica et Traumatologica Turcica publishes clinical and basic research articles, case reports, personal clinical and technical notes, systematic reviews and meta-analyses and letters to the Editor. Proceedings of scientific meetings are also considered for publication. The target audience of the journal includes healthcare professionals, physicians, and researchers who are interested or working in orthopedics and traumatology field, and related disciplines.
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