Potential diagnostic role of circulating MiRNAs in colorectal cancer.

IF 3.5 3区医学

International Journal of Immunopathology and Pharmacology Pub Date : 2023-01-01 DOI:10.1177/03946320221144565

Dina A Abo-Elela, Ahmed Mh Salem, Menha Swellam, Marwa Ga Hegazy

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引用次数: 4

Abstract

Objectives: Colorectal cancer (CRC) is the third most common and fourth most deadly cancer worldwide despite its various screening method. Thus, the search for novel and better markers is continuous. This study aimed to assess the combined expression levels of miR-133a, miR-574-3p, and miR-27a in early diagnosis of colorectal cancer in comparison to traditional tumor markers (CEA and CA19.9).

Methods: miR-133a, miR-574-3p, and miR-27a were assessed in sera of 120 participants categorized into healthy control group (n = 20), benign group (n = 30) and malignant group (n = 70) using real-time PCR.

Results: miR-133a, miR-574-3p, and miR-27a expressions showed significant difference among different staging, grading and tumor size of CRC. The sensitivities of the three miRNAs whether combined or individually used were better than routinely used tumor markers (CEA and CA19.9) leading to more accurate and faster diagnosis of CRC.

Conclusion: Synergetic detection of miRNA-133a, miRNA-574-3p, and miRNA-27a may serve as better noninvasive biomarkers with higher combined sensitivity for early diagnosis of CRC than individual detection of miRNAs.

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循环mirna在结直肠癌中的潜在诊断作用。

目的:结直肠癌(CRC)是全球第三大最常见和第四大最致命的癌症，尽管其筛查方法多种多样。因此，寻找新的和更好的标记是持续的。本研究旨在评估miR-133a、miR-574-3p和miR-27a在结直肠癌早期诊断中的联合表达水平，并与传统肿瘤标志物(CEA和CA19.9)进行比较。方法:采用实时荧光定量PCR检测健康对照组(n = 20)、良性组(n = 30)和恶性组(n = 70) 120例患者血清中miR-133a、miR-574-3p和miR-27a的含量。结果:miR-133a、miR-574-3p、miR-27a的表达在结直肠癌不同分期、分级及肿瘤大小之间存在显著差异。这三种mirna无论是联合使用还是单独使用的敏感性都优于常规使用的肿瘤标志物(CEA和CA19.9)，从而更准确、更快速地诊断结直肠癌。结论:协同检测miRNA-133a、miRNA-574-3p和miRNA-27a可能比单独检测miRNA-133a、miRNA-574-3p和miRNA-27a作为早期诊断结直肠癌的更好的无创生物标志物，其联合敏感性更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Immunopathology and Pharmacology Immunology and Microbiology-Immunology

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期刊介绍： International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.