Recruitment of myeloid‑derived suppressor cells and regulatory T‑cells is associated with the occurrence of acute myocardial infarction.

IF 2.3 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Mingqiang Zhang, Xiaohu Shi, Jingquan Zhao, Wenjia Guo, Jie Zhou
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引用次数: 0

Abstract

The roles of myeloid-derived suppressor cells (MDSCs) and regulatory T-cells (Tregs) in acute myocardial infarction (AMI) remain elusive. The present study aimed to analyze the proportions of the granulocytic and monocytic populations of MDSCs (G-MDSCs and M-MDSCs, respectively), and Tregs in the peripheral blood mononuclear cells (PBMCs) of patients with AMI. The present study recruited 34 patients with AMI and 37 healthy controls without clinical signs of myocardial ischemia. PBMCs were isolated from the peripheral blood samples of patients with AMI within 24 h following admission to the hospital and from those of the healthy controls during a physical examination. Two subsets of MDSCs, G-MDSCs (CD15+CD33+CD11b+CD14-HLA-DRlow) and M-MDSCs (CD14+CD15-CD11b+HLA-DRlow), and Tregs (CD3+CD4+CD25highCD127low T-cells) in the PBMCs derived from the patients with AMI and healthy controls were analyzed using flow cytometry. The effects of MDSCs derived from patients with AMI on naïve CD4+ T-cells were examined in the co-culture system. The results revealed that the proportions of G-MDSCs and M-MDSCs were higher in the peripheral blood of patients with AMI than in that of the healthy controls. The patients with AMI had significantly higher numbers of programmed death-ligand (PD-L)1- and PD-L2-positive G-MDSCs and M-MDSCs compared with the healthy controls (P<0.05). The MDSCs could acquire a granulocytic phenotype following AMI, and the G-MDSCs and M-MDSCs would be more likely to express PD-L2 and PD-L1, respectively. The ratios of Tregs to CD4+ T-cells and PD-1+ Tregs in the peripheral blood of patients with AMI were significantly higher than those in the healthy controls (P<0.05). The results of flow cytometry demonstrated an increase in the numbers of inducible Tregs in the co-culture system with the G-MDSCs derived from patients with AMI compared with the G-MDSCs derived from the healthy controls (P<0.01). On the whole, the findings presented herein demonstrate the accumulation of MDSCs, and the upregulation of PD-L1 and PD-L2 expression on the surface of MDSCs in patients with AMI. MDSCs can induce the expansion of Tregs by binding PD-1 on the surface of Tregs, thus playing a crucial role in AMI.

髓源性抑制细胞和调节性T细胞的募集与急性心肌梗死的发生有关。
髓源性抑制细胞(MDSCs)和调节性t细胞(Tregs)在急性心肌梗死(AMI)中的作用尚不清楚。本研究旨在分析AMI患者外周血单核细胞(PBMCs)中粒细胞和单核细胞群MDSCs(分别为G-MDSCs和M-MDSCs)和Tregs的比例。本研究招募了34例AMI患者和37例无心肌缺血临床症状的健康对照。从AMI患者入院后24小时内的外周血样本和体检期间的健康对照中分离出pbmc。采用流式细胞术分析急性心肌梗死患者和健康对照组外周血中两个MDSCs亚群G-MDSCs (CD15+CD33+CD11b+CD14-HLA-DRlow)、M-MDSCs (CD14+CD15-CD11b+HLA-DRlow)和Tregs (CD3+CD4+CD25highCD127low t细胞)。在共培养系统中检测AMI患者来源的MDSCs对naïve CD4+ t细胞的影响。结果表明,心肌梗死患者外周血中G-MDSCs和M-MDSCs的比例高于健康对照组。AMI患者的程序性死亡配体(PD-L)1和pd - l2阳性的G-MDSCs和M-MDSCs的数量明显高于健康对照组(AMI患者外周血P+ t细胞和PD-1+ Tregs显著高于健康对照组)
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来源期刊
Biomedical reports
Biomedical reports MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
4.10
自引率
0.00%
发文量
86
期刊介绍: Biomedical Reports is a monthly, peer-reviewed journal, dedicated to publishing research across all fields of biology and medicine, including pharmacology, pathology, gene therapy, genetics, microbiology, neurosciences, infectious diseases, molecular cardiology and molecular surgery. The journal provides a home for original research, case reports and review articles.
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