Electroosmotic flow across nanopores for single-molecule protein sequencing

IF 33.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nature biotechnology Pub Date : 2023-09-18 DOI:10.1038/s41587-023-01976-5

引用次数: 0

Abstract

By using fixed charges to engineer a strong electroosmotic flow, we achieve the unidirectional transport of natural polypeptides across nanopores. Our approach enables native proteins to be transported enzymatically and non-enzymatically in the absence of denaturant and electrophoretic tags, with potential applications for protein sequencing.

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用于单分子蛋白质测序的穿过纳米孔的电渗流。

通过使用固定电荷来设计强大的电渗流，我们实现了天然多肽在纳米孔中的单向传输。在没有变性剂和电泳标签的情况下，我们的方法可以实现原生蛋白质的酶促和非酶促传输，并有望应用于蛋白质测序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature biotechnology 工程技术-生物工程与应用微生物

CiteScore

63.00

自引率

1.70%

发文量

382

审稿时长

3 months

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